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Rapid screening of protein interactions with histone peptide arrays

EpiCypher's EpiTitan™ Histone Peptide Arrays 

EpiTitan™ Histone Peptide Arrays are designed for rapid and high-throughput screening of effector protein, antibody and enzyme interactions with a comprehensive library of combinatorially modified and biotinylated histone peptides imobilized on a streptavidin-coated glass slide.

EpiCypher's EpiGold™ Histone Peptide Arrays Workflow

 

 

 

 

 

 

 

 

 

EpiTitan™ Histone Peptide Array applications & formats

 
EpiCypher offers the only histone peptide array that can be analyzed using fluorescence scanning or standard ECL detection methods.

 

2 formats available:

 
All the peptides spotted one the arrays can be purchased separately for further analysis. Contact us to discuss about your peptide(s) of interest.

Typical results with Epicypher's™ Histone Peptide Arrays

EpiCypher's Histone Peptide Array Data-Fluorescence
 
EpiGold Histone Peptide Array Fluorescent detection
 
Scan of an array used to detect the binding specificity of  Histone H3 K4Me3 Antibody (cat. nr. 23613-0004). The patented spotting tracer appears green, and H3 K4Me3 binding is shown in red. Since peptides on the array are spotted 24 times in sets of three (8 sets for each peptide), authentic binding events appear as three adjacent red dots, indicating that the antibody has bound all three of the same peptide in the set.
 
EpiCypher's Histone Peptide Array Data-ECL
 
EpiGold™ Histone Peptide Array Data-ECL
 

Scan of an array used to detect the binding specificity of  Histone H3 K4Me3 Antibody (cat. nr. 23613-0004) ECL detection visualized by 30-second exposure to X-ray film.

 

EpiCypher's Histone Peptide Arrays have been used successfully with dozens of proteins...

A few publications:

  • Cai L et al (2013). An H3K36 Methylation-Engaging Tudor Motif of Polycomb-like Proteins Mediates PRC2 Complex Targeting. Mol Cell 49: 571-582.
  • Rothbart SB et al (2013). Multivalent histone engagement by the linked tandem Tudor and PHD domains of UHRF1 is required for the epigenetic inheritance of DNA methylation. Genes Dev 27: 1288-1298.
  • Rothbart SB, Krajewski K, Strahl BD, Fuchs, SM (2012). Peptide microarrays to interrogate the histone code. Methods Enzymol 512: 107-135.
  • Rothbart SB et al (2012). Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation. Nat Struct Mol Biol 19: 1155-1160.
  • Fuchs SM, Krajewski K, Baker RW, Miller VL, Strahl BD (2011). Influence of combinatorial histone modifications on antibody and effector protein recognition. Curr Biol 21: 53-58.

Contact us about your effector / enzyme binding sudies

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