tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences
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TALEN by tebu-bio

TAL effectors (TALEs) are today’s last method to create site-specific gene-editing tools by fusing target sequence-specific TAL effectors to nucleases (TALENs), transcription factors (TALE-TFs) and other functional domains. These fusion proteins can recognize and bind chromosome target sequences specifically then edit the genes according to their function: knockout, knockin (with donor plasmid), modification, activation, repression and more.

Unlike zinc fingers triplet recognition, TAL effector domains recognize single nucleotides, which allows researchers to be able to specifically target whatever sequence they want.

Advantages

TALEN

TAL effector nuclease (TALEN) contains a TALE DNA binding domain fused to the FokI nuclease. Two TALENs must bind on each side of the targeted site for FokI to dimerize and cut at a specific site. TALENs induce a site-specific double-strand break (DSB), which the cellular repair mechanism of non-homologous end joining (NHEJ) can then reconnect.
Alternatively, an exogenous double-stranded donor DNA fragment can be introduced into the genome at the DSB by homologous recombination (HR).

TALENs have been used to generate stably modified human embryonic stem cell and induced pluripotent stem cell (IPSCs) clones, and to generate knockout organisms in rat, C.elegans, and zebrafish.

Please contact us if you want more information about our TALEN design service.

TALE-TF

A key application for TALEs is the targeted activation and repression of target genes in cells by fusing trans-activation domains to TALE DNA binding domains. The TALE-TF construct is a powerful tool to selectively modulate gene expression in eukaryotic cells with exquisite specificity. The TALE-TF contains a TALE DNA binding domain fused to the VP64 transcription.

Please contact us if you want more information about our TALE-TF design service.

Genome TALER human AAVS1 safe harbor kit

This kit is designed to specifically transfer your gene of interest, selection marker or other genetic element into the AAVS1 safe harbor site in human chromosome 19 via TALEN mediated homologous recombination.  The kit includes one pair of AAVS1 TALENs, one donor cloning vector or one donor clone, one positive control clone and one knock-in PCR verification kit.

 

Please contact us if you want more information about our kit and estimated price according to donor plasmid.

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