Sumo-based expression systems

How to improve the solubility of protein expression

To maximize the yield of soluble and functional proteins produced in E. coli, Yeast, Insect or mammalian cells, the SUMO-based protein expression systems allow the improvement of recombinant proteins solubility.

This system utilizes unique features of SUMO functioning as both a chaperonin and as an initiator of protein folding to dramatically improve the solubility and level of expression of the protein of interest.

SUMO protease efficiently and robustly remove SUMO tags precisely at the junction of the C-terminus of the SUMO and the N-terminus of the protein releasing it with the desired N-terminal amino acid (with the exception of proline).

Both the SUMO tags and the SUMO proteases have His6 tags making their subsequent removal fast and easy.

3 different SUMO expression systems:

SUMOpro:

• Original SUMO System - Smt3 from yeast
• Only work in prokaryotic expression sustem
• Cleaved by SUMO Protease 1 (Ulp1)

SUMOstar

• Ideal for eukaryotic systems
• Mutated and thus not recognized by native SUMO proteases
• Cleaved by SUMOstar Protease 1

SUMOpro3

• Based on the Huamn SUMO-3 protein which is resistant to desumoylases found in eukaryotic cells
• Cleaved by SUMO Protease 2

Which suitable system for your type of expression?

E. coli

• SUMOstar kit: available either ampicillin or kanamycin resistance selectable markers
• SUMOpro Kit: available either ampicillin or kanamycin resistance selectable markers
• SUMOpro3 Kit:available either ampicillin or kanamycin resistance selectable markers

Mammalian

• pM-Mammalian Intracellular SUMOstar Kit
• pM-Mammalian Secretory SUMOstar Kit

Insect

• Insect Intracellular Expression: pI-Insect (BEVS) SUMOstar Intracellular Expression Kit
• Insect Secretory Expression: pI-Insect (BEVs) Secretory SUMOstar Expression Kit

Yeast

• Yeast Intracellular Expression (Saccharomyces): pY-Saccharomyces Intracellular SUMOstar Kit
• Yeast Secretory Expression (Pichia): pY-Pichia Secretory SUMOstar Expression Kit

Sumo tag lifting with SUMO Proteases!

• Recognition of the tertiary structure of SUMO for specific digestion and removal of SUMO tags
• Robust recombinant protease
• No cleavage within the fused protein of interest
• Highly active: 1U of SUMO Protease cleaves >90% of SUMO Protease Control Protein in 1 hour (30°C)