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Human Smooth Muscle Cell Lines

The HITB5, HITC6 and HITD5 clones were generated from primary cultures of human smooth muscle cells prepared from internal thoracic artery. These cells assume a proliferative, motile phenotype when cultured in M199 media in the presence of 10% FBS. When serum deprived the cells no longer proliferate but assume an elongated spindle-shaped morphology with suppressed motility. The serum deprived cells are also seen to contract in vitro in response to the vasoactive hormones histamine and angiotensin II.

These cell lines may be valuable for clarifying our understanding of SMC phenotype switching and restructuring of the vessel wall. Additionally, these cell lines are ideal for studies involving heart disease, stroke, angiogenesis and vasculogenesis, drug development, toxicity, cell-cell interactions, wound healing and cancer therapy.

Figure 1. Phase-contrast images of HITB5 smooth muscle cells cloned from adult internal thoracic artery. (a,b) HITB5 cells grown in M199 media with 10% FBS. (c,d) HITB5 cells 3 days after serum withdrawal showing an elongated and spindle-shaped morphology. (Shaohua Li et al (1999). Circulation Research. 85: 338-348.)

Figure 2. Phase-contrast images of HITC6 smooth muscle cells before (a) and after (b) the application of Angiotensin II (1 mmol/L) showing contraction. (Shaohua Li et al (2001). Circulation Research. 89: 517-525.)