Oris Cell Migration

Adherent cell migration without cell culture inserts

The Oris™ Cell Migration Assay* is designed with a unique cell seeding stopper, detection mask, and stopper tool. There is no Transwell® membrane insert needed. This unique plate design generates highly reproducible results using a microscope, digital imaging system or fluorescence plate reader. Simply adhere the seeded cells in each well, remove the stopper to create a detection zone, and measure the migration (see Figure 1 in the gallery).

Oris™ assays are available with tissue culture treated plates, Collagen I coated plates, Fibronectin coated plates, or a TriCoated version that contains all 3 surfaces.

Wound healing assays are normally performed using a scratch assay, which has high variability. The Oris™ Cell Migration Assay is an ideal alternative to the scratch assay since the cell seeding stoppers create a consistent detection zone to monitor 2-D closure.

Features & Benefits of the Oris™ Cell Migration Assay

• Membrane-free migration - no cumbersome cell culture inserts; stain and view cells in the same well.
• Reproducible results - the unique design provides well-to-well CV's < 12%
• Preserve cell morphology - changes in cell structure can be monitored in real time
• Versatile - analyze data using multiple probes in a single well using a microscope, digital imager, or fluorescence plate reader
• Flexible - design kinetic or endpoint based cell migration assays without the use of special instrumentation

Oris™ Cell Migration Assay Video

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* Patent Pending.
Oris™ is a trademark of Platypus Technologies, LLC