Rat and Mouse Skin Microsomes & S9
See the related productsMany drug metabolizing enzymes of the liver and other visceral organs are also expressed in the skin. The skin possesses a broad spectrum of xenobiotic metabolizing enzymes including cytochromes P450, flavin monooxygenases, esterases/amides, UDP-glucuronosyltransferases, glutathione S-transferases and sulfotransferases. Specific activities of these cutaneous enzymes are typically less than 10% of those found in the liver, yet they may play a significant role in the biotransformation of xenobiotics absorbed and/or delivered through the skin.
Characterisation provided
- NADPH-cytochrome c reductase activity
- Testosterone 6β-hydroxylation
- 4-Methylumbelliferone glucuronidation
- Clopidogrel hydrolysis
- Methylprednisolone 21-hemisuccinate hydrolysis
- Animal information and husbandry
Microsomes
Suspension buffer: 250mM sucrose
S9 Fraction
Suspension buffer: 50mM TRIS HCl (pH 7.4 at 4°C) containing 150mM KCl and 2 mM EDTA
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