Rat and Mouse Skin Microsomes & S9

Many drug metabolizing enzymes of the liver and other visceral organs are also expressed in the skin. The skin possesses a broad spectrum of xenobiotic metabolizing enzymes including cytochromes P450, flavin monooxygenases, esterases/amides, UDP-glucuronosyltransferases, glutathione S-transferases and sulfotransferases. Specific activities of these cutaneous enzymes are typically less than 10% of those found in the liver, yet they may play a significant role in the biotransformation of xenobiotics absorbed and/or delivered through the skin.

Characterisation provided

• NADPH-cytochrome c reductase activity
• Testosterone 6β-hydroxylation
• 4-Methylumbelliferone glucuronidation
• Clopidogrel hydrolysis
• Methylprednisolone 21-hemisuccinate hydrolysis
• Animal information and husbandry

Microsomes

Suspension buffer: 250mM sucrose

S9 Fraction

Suspension buffer: 50mM TRIS HCl (pH 7.4 at 4°C) containing 150mM KCl and 2 mM EDTA