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Extracellular matrix components detection

Finding the right product suited to your application is important - to help you in choosing, we have created the following Product Guide linked to detection of extracelluar matrix components. Our technical specialists will also be pleased to help you with your selection.

Depending on which extracellular matrix components you would like to study, tebu-bio brings various high quality assays for use with mammalian cells, tissues and fluids.Simply click on any of the topics below to access full information:

Collagen quantitative measurement:

Soluble collagen:

The Sircol Assay (Cat nr S1000) is a dye-binding method designed for the analysis of acid and pepsin-soluble collagens.

Collagen IV

Elastin:

Fastin Elastin Assay Kit (Cat nr F2000) is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials (publications list):

Proteoglycan:

Blyscan Glycosaminoglycan Kit (Cat nr B1000) is a quantitative dye-binding assay for analysis of sulfated proteoglycans & glycosaminoglycans (sGAG).
The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples. The dye label used in the assay is 1, 9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains ().

Test material can be assayed directly when present in a soluble form, or following papain extraction from biological materials as:

 

Publications in scientific literature describing the use of this assay with various cell types

Hyaluronic Acid:


Hyaluronan ELISA Kit (Cat nr K-1200-0001) is a quantitative enzyme-linked immunoassay designed for the in vitro measurement of HA levels in human or animal biological fluids (blood, serum, urine, diffusate, synovial fluid). This simple protocol is a standard ELISA format and requires 3 hours only. This assay is a competitive ELISA assay in which the colorimetric signal is inversely proportional to the amount of HA present in the sample.

Enzymes involved in destruction of extracellular matrix

Serine proteases
Metalloproteases

Our unique protein search engine is at your fingertips! And to make it even easier, you can fine-tune your search by filtering with a selection of criteria:

 Try our protein search engine now and discover how easy it is to find your proteins of interest!