HapiCell™ Transfection Toxicity Removal Cocktail

HapiCell™ Transfection Toxicity Removal Cocktail is a proprietary formulation for effectively eliminating DNA or siRNA transfection mediated toxicity.

The HapiCell™ Transfection Toxicity Removal Cocktail was developed to contain a mixture of several chemicals which were confirmed to effectively remove DNA/siRNA transfection mediated cytotoxicity. Incubation of sensitive cells (e.g., SaoS-2, HUVEC, primary kerotinocytes, etc) with HapiCell™ Toxicity Removal Reagent for 2 minutes at RT was confirm to completely remove the transfection related toxicity which otherwise kills the cells. The cocktail was used for chemical transfection only, not for electroporation. HapiCell™ Transfection Toxicity Removal Cocktail, 2.5 ml (x50 stock solution), is sufficient to treat 125 wells for 24-well plate and 63 wells for 6-well plates respectively.

Why use HapiCell?

• Removes transfection mediated toxicity after 2 minutes incubation at RT
• Non-toxic to cells
• Maintains cell viability
• Improves transfection efficiency
• Easy to use

HapiCell™ effectively removes transfection mediated toxicity

 

An example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on mouse skin fibroblast. A DNA encoding GFP was delivered to mouse skin fibroblasts by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection.
 

 

An example showing complete removal of transfection toxicity after treatment with HapiCell™ Transfection Toxicity Removal Cocktail on primary mouse kerotinocytes. A DNA encoding GFP was delivered to primary mouse kerotinocytes by Lipofectamine 2000 in absence of serum. Serum-containing medium was changed back 5 hours post transfection followed 2 minutes incubation with (left panel) or without (right panel) HapiCell™ Transfection Toxicity Removal Cocktail. The cell viability was checked under microscope 24 hours post transfection.