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Phosphorylation focus

tebu-bio tool box          

Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation turns many protein enzymes on and off, causing or preventing the mechanisms of diseases such as cancer and diabetes.
Protein and lipid phosphorylation in particular play a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research.

 

tebu-bio offers the broadest range of tools to work on phosphorylation, whenever you want a ready-to-use protocol or an enzyme to develop your own assay.
  

Protein phosphorylation

Monitor kinase activity

PKA activity assay

Develop kinase & phosphatase activity assays:

Recombinant enzymes and modulating molecules of reference (browse our Proteins and Biomolecules search engines)


Profile phosphorylation status of a family of proteins:

Phosphorylation antibody arrays for simultaneously detecting the relative level of Tyrosine phosphorylation of Human kinases (ex Receptor Tyrosine Kinases (RTKs) and ERK)

Read-out: Chemiluminescence or fluorescence laser scanner

Measure phosphorylation of proteins inside cells:

Cell-based ELISA determine ratio of selected phosphorylated protein versus total (ex: STAT5).
Read-out: ELISA colorimetry


Measure phosphorylation of proteins in cell lysate

PhosphoELISA : Ready-to-use ELISA kits to measure selected phosphorylated protein (or phospho- vs pan protein).
Read-out: ELISA colorimetry


Determine expression status by WB, IP, IF, IHC…:

p-Antibodies and their corresponding total protein antibodies: just type the name of your target in our search engine and then refine Primaries on Modification  = Phospho

 

Quantify the activity of an unknown enzyme (or for which substrates are unknown):

Determine the liberation or capture of phosphates with complete kits

 

 

 

Lipid phosphorylation


Develop lipid kinase or lipid phosphatase activity assays


Recombinant enzymes, substrate (lipid) and modulating molecules of reference (browse our Proteins and Biomolecules search engines)


Modulators and drug candidates screening, study enzyme kinetic in vitro and test the effect of compounds

Ready to use ELISA (PI3K, LPA, S1P)


Study enzyme activity and pharmacology of compounds in cells