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Microtubule / tubulin in vivo kit

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Introduction

The most reproducible and accurate method of determining the amount of microtubule content versus free-tubulin content in a cell population is to use western blot quantitation of microtubule and free-tubulin cellular fractions.  The general approach is to homogenize cells in microtubule stabilization buffer, followed by centrifugation to separate the microtubules from free-tubulin pool. Then the fractions are separated by PAGE and tubulin is quantitated by western blot. The final result gives the most accurate method of determining the ratio of tubulin incorporated into the cytoskeleton versus the free-tubulin found in the cytosol. This kit contains all the reagents to perform this assay.

Kit contents

The kit contains sufficient materials for 30-100 assays depending assay setup and includes reagents for positive and negative controls. The following components are included:

   1. Lysis and Microtubule stabilization buffer
   2. GTP (Cat nr BST06)
   3. ATP (Cat nr BSA04)
   4. Protease inhibitor cocktail
   5. Microtubule enhancing control solution
   6. Microtubule depolymerization control solution
   7. Control Tubulin Standard (Cat nr TL238)
   8. Tubulin monoclonal antibody (Cat nr ATN01)
   9. SDS sample buffer (5 x)
  10. DMSO
  11. Manual with detailed protocols and extensive troubleshooting guide

Equipment needed

Product Uses Include

Examples of publications where this product was used


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