Small GTPase Product Guide

Finding the right product suited to your application is important - to help you in choosing, we have created the following Product Guide. Our technical specialists will also be pleased to help you with your selection.

Simply click on any of the topics below to access full information:

• Activation assays
• Inhibitors and activators
• Purified proteins
• Antibodies
• Total RhoA ELISA Kit
• Phosphate quantification

Activation Assays

G-LISA:
A revolutionary new way of doing Small G-protein Activation Assays!

G-LISA assays are simple, fast (<3 hours), require only small amounts of sample (5-25 µg cell protein), yield quantitative and accurate data and are suitable for high thoughput screens. Learn more here.

• Cdc42 G-LISA Activation Assay, colorimetric format
• Rac1,2,3 G-LISA Activation Assay, colorimetric format
• RhoA G-LISA Activation Assay, colorimetric format
• RhoA G-LISA Activation Assay, luminescence format
• Rac1 G-LISA Activation Assay, luminescence format
• Rac1 G-LISA Activation Assay, colorimetric format
• RalA G-LISA Activation Assay, colorimetric format
• Ras G-LISA Activation Assay, colorimetric format
• Arf1 G-LISA Activation Assay, colorimetric format
• Arf6 G-LISA Activation Assay, colorimetric format

 Pull-down

• Rac activation assay
• Ras activation assay
• RhoA activation assay

RhoGAP& RhoGEF activity assays
The Ras superfamily of small GTPases serve as binary switches cycling between a GDP-bound “OFF state” and a GTP-bound “ON state”, which mediates signals downstream. In cells, the cycling between the two states is mainly controlled by two types of regulatory proteins: the activating Guanine nucleotide Exchange Factors (GEFs) and the inactivating GTPase Activating Proteins (GAPs).

• RhoGAP assay
• RhoGEF exchange assay

Inhibitor & Activators

Rho Inhibitor

• C3 Transferase cell permeable
• C3 transferase

Activators

• Rho Activator
• Rac and Cdc42 Activator

Purified proteins

GTPases

• Cdc42 Human GST
• Cdc42 Human His
• Cdc42 Human His - Constitutively active
• Cdc42 Human GST - Dominant negative

• H-RasHuman His

• Rac1 Human GST
• Rac1 Human His
• Rac1 Human His - Constitutively active
• Rac1 Human GST - Dominant negative

• Ran Human His
• Ran Human His - Constitutively active
• Ran Human His - Dominant negative

• Rap1aHuman His
• Rap1b Human His

• RhoA Human GST
• RhoA Human His
• RhoA Human His - Constitutively active
• RhoC Human His

• GTP-gamma-S
  non-hydrolyzable analog of GTP, for preparing "active conformation" of G-proteins

Effector fragments

• PAK p21-binding domain protein GST
• PAK p21-binding domain protein GST - On agarose beads
• Raf Ras-binding domain protein GST - On agarose beads
• RanBP1Human His
• Rhotekin Rho-binding domain protein GST
• Rhotekin Rho-binding domain protein GST - On agarose beads

GTPase activating protein (GAPs)

• p50RhoGAP Human GST
• p50RhoGAP Human GST - Catalytic domain
   

Guanine nucleotide dissociation inhibitors (GDIs)

• Rho guanine nucleotide dissociation inhibitor protein-1 GST
   

Guanine nucleotide exchange factors (GEFs)

• Dbs RhoGEF domain protein His

Antibodies

• Anti Cdc42 monoclonal antibody
• Anti Rac1 monoclonal antibody
• Anti RhoA monoclonal antibody
• Anti Cofilin rabbit polyclonal
• Anti Profilin rabbit polyclonal

Total RhoA level measurement

Faster, easier and more robust than conventional Western blot, the RhoA ELISA kit is designed as a highly selective sandwich ELISA format for RhoA measurement in cell and tissue samples in less than 5 hours.

• Extremely sensitive - detection of as little as 1 ng of total RhoA
• Only 5-10 µg of total cell or tissue protein needed
• Multi-species detection (Human, Mouse, Porcine)
• Pre-coated plate for high reproducibility
• Companion for our RhoA G-LISA™ kit
   

Phosphate quantification

Cytoskeleton Inc. has introduced the largest range of highly robust phosphate assays currently available

• Learn more on our dedicated page