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Advanced Protein Assay

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Protein assay reagents from Cytoskeleton Inc

Cytoskeleton, Inc. offers two different protein assays, the Advanced Protein Assay (ADV01) and the Precision Red Advanced Protein Assay (ADV02). Both these assays combine many great features making them advantageous over conventional protein assays:

Need for sensitivity?

ADV01 is a protein assay with very high sensitivity, making it ideal for uses such as measuring protein concentration in:

Need for broad linear range?

ADV02 is a protein assay vwith a very broad linear range. This reagent is so reproducible with a wide range of proteins and protein concentrations that it is employed to accurately determine the concentration in all of Cytoskeleton, Inc's protein products. It is ideal for uses such as measuring protein concentrations in:

 

Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA™ small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.

 

Chemical tolerance for the ADV01 & ADV02

    ADV01  ADV02 
Chemical group Chemical name
Tested concentration that does not alter protein assay color*
Tested concentration that does not alter response to protein*
Tested concentration that does not alter protein assay color*
Tested concentration that does not alter response to protein*
Buffers Tris pH 8.0
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  Hepes pH 8.0
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  Pipes pH 7.0
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  Potassium phosphate pH7.0
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  Sodium bicarbonate pH9.5
>1.0 M
>1.0 M
>1.0 M
>1.0 M
   
 
 
 
 
Reducing agents BME
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  DTT
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  monothioglycerol
>1.0 M
>1.0 M
>1.0 M
>1.0 M
   
 
 
 
 
Denaturants 8M urea
100%
100%
100%
100%
  5M guanidine-HCl
100%
100%
100%
100%
   
 
 
 
 
Divalent cations MgCl2
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  CaCl2
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  NiCl2
>1.0 M
>1.0 M
>0.1 M
>0.1 M
   
 
 
 
 
Chelating agents EDTA
>1.0 M
>1.0 M
>1.0 M
>1.0 M
  EGTA
>1.0 M
>1.0 M
>1.0 M
>1.0 M
   
 
 
 
 
Detergents SDS
5.00%
0.50%
0.10%
0.10%
  NP40
0.50%
0.20%
10%
1.0%
  Triton X-100
0.20%
0.20%
10%
0.50%
  Tween 20
1.00%
0.50%
10%
1.0%
  CHAPS
0.50%
0.10%
1.0%
0.1%
  Ampholytes
0.50%
0.10%
1.0%
0.1%
   
 
 
 
 
Solvents DMSO
100%
100%
100%
100%
  DMF
100%
100%
100%
100%
  Ethanol
100%
100%
100%
100%
  Methanol
100%
100%
100%
100%
   
 
 
 
 
Antifoaming agent Antifoam-C
30%
30%
10%
10%
   
 
 
 
 
Acids Hydrochloric acid
>1.0 M
>1.0 M
>1.0 M
>0.75 M
  Perchloric acid
>1.0 M
>1.0 M
>1.0 M
>0.75 M
  Trichloric acid
>1.0 M
>1.0 M
>1.0 M
>0.50 M
  Nitric acid
>1.0 M
>1.0 M
>1.0 M
>0.75 M
  Sulfuric acid
>1.0 M
>1.0 M
>1.0 M
>0.25 M

*using 10 µl protein solution in 1.0 ml protein assay reagent

Table 1. Chemical tolerance for the ADV01 and ADV02 protein assay reagents

 


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