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HiLyte Fluorescent dyes

AnaSpec’s proprietary HiLyte Fluor™ dyes are a series of excellent fluorescent labeling dyes with fluorescence emissions that span the full visible and near infrared spectrum.

Facts and benefits

The hydrophilic property of most of the HiLyte Fluor™ dyes makes conjugations to protein easier, minimizing the use of organic solvents. The resulting conjugates are relatively resistant to aggregation and precipitation during storage. HiLyte Fluor™ dyes also have better labeling performance than the classic fluorescent labeling dyes such as FITC and rhodamines.

Superior fluorescent properties

Versatility

Coupling flexibility

 

 HiLyte Fluor™ Dye  Ex (nm) Em (nm)   Extinction Coefficient
(M-1cm-1)
 Fluorescence Quantum Yield  Fluorescence Life Time 
 HiLyte Fluor™ 405  404  428  35,000  0.54 Not available
 HiLyte Fluor™ 488  497  525  70,000  0.91  4.1
 HiLyte Fluor™ 555  550  566  150,000  0.10  0.3
 HiLyte Fluor™ 594  593  616  80,000  0.90  4.2
 HiLyte Fluor™ 647  649  672  250,000  1.0  1.0
 HiLyte Fluor™ 680  688  700  190,000  1.1  1.1
 HiLyte Fluor™ 750  750  782  275,000  0.7  0.7