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PREDEASY ATPase Assay Kits

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It is a Unique Product Platform for measuring the ATPase dependent Drug - ABC membrane transporter interaction in a "Ready to Assay" All-in-One Package! The Kit not only contains all reagents to conduct the assay but also delivers major technical improvements over the currently available conventional products.

Predeasy vs. conventional assays

Kits available

PREDEASY ATPase Kits are available for all relevant membrane transporters from SOLVO's ATPase portfolio.

 Product name  Intended use  Reference substrate
 SB-MDR1-PREDEASY™-ATPase  ATPase activation and inhibition  Verapamil
 SB-ratMdr1b- PREDEASY™ -ATPase  ATPase activation and inhibition  Verapamil
 SB-MRP1- PREDEASY™ -ATPase  ATPase activation and inhibition  NEM-GS
 SB-MRP2- PREDEASY™ -ATPase  ATPase activation and inhibition  Sulfasalazine
 SB-MRP3-PREDEASY™ -ATPase  ATPase activation and inhibition  Benzbromarone
 SB-BCRP-HAM- PREDEASY™ -ATPase  ATPase activation and inhibition  Sulfasalazine
 SB-defBCRP- PREDEASY™ -ATPase  ATPase activation and inhibition   -
 SB-defMRP-PREDEASY™ -ATPase  ATPase activation and inhibition  -

 

3 sizes for more flexibility

PREDEASY ATPase Assay Kits are available in three sizes 1, 3 and 10 (96 well) plate sizes. According to the suggested assay layout on one plate both activation and inhibition tests can be done in duplicates with 8 different concentrations of the test drug and including all relevant controls.

PREDEASY ATPase Kit composition

Equipment and materials needed

Water bath, automatic pipettes, multichannel-pipette, 96 well microtiter plates, microplate reader with absorbance filter suitable for measuring OD between 590 and 630 nm, MilliQ water, DMSO, 2 ml, 5ml & 10 ml tubes.


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