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My accountHuman Bactosomes
Bacterial membranes containing the human cytochrome P450s coexpressed with human NADPH-cytochrome P450 reductase.
Bactosomes advantages
- High batch to batch reproducibility
- High CYP450 enzymatic levels
-
Intensive batch characterization
- Km
- Vmax
- Linearity (time & enzyme)
Bactosomes can be used in conjunction with the Simcyp simulator.
Phase I enzymes available
CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2A13 CYP2B6
CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1
CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2
Phase II enzymes available
Sulfotransferases Glutathione S-transferases Aldehyde oxidase
UDP-glucuronosyltransferases Carboxylesterase Aldehyde dehydrogenase
High or low reductase?
Bactosomes are available in High Reductase & Low reductase formats.
| High Reductase | Low Reductase | |
| Characteristics | ||
| Higher activity | Lower activity | |
| Shorter time linearity | Longer time linearity | |
| May not reflect in vivo | Better reflects in vivo | |
| Applications | ||
| Reaction phenotyping | Some reaction phenotyping | |
| Metabolite profiling | High throughput inhibition screening | |
| ID minor metabolites | ||
| Some inhibition screening |
Note: ‘R’ = high reductase, ‘LR’ = low reductase, except for CYP2C9 (‘HR’ = high reductase, ‘R’= low reductase’)
