Human Bactosomes
See the related productsBacterial membranes containing the human cytochrome P450s coexpressed with human NADPH-cytochrome P450 reductase.
Bactosomes advantages
- High batch to batch reproducibility
- High CYP450 enzymatic levels
-
Intensive batch characterization
- Km
- Vmax
- Linearity (time & enzyme)
Bactosomes can be used in conjunction with the Simcyp simulator.
Phase I enzymes available
CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2A13 CYP2B6
CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1
CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2
Phase II enzymes available
Sulfotransferases Glutathione S-transferases Aldehyde oxidase
UDP-glucuronosyltransferases Carboxylesterase Aldehyde dehydrogenase
High or low reductase?
Bactosomes are available in High Reductase & Low reductase formats.
High Reductase | Low Reductase | |
Characteristics | ||
Higher activity | Lower activity | |
Shorter time linearity | Longer time linearity | |
May not reflect in vivo | Better reflects in vivo | |
Applications | ||
Reaction phenotyping | Some reaction phenotyping | |
Metabolite profiling | High throughput inhibition screening | |
ID minor metabolites | ||
Some inhibition screening |
Note: ‘R’ = high reductase, ‘LR’ = low reductase, except for CYP2C9 (‘HR’ = high reductase, ‘R’= low reductase’)
See the related products