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Oris Invasion & Detection Assay

View Cell Invasion in 3-D!

The Oris Cell Invasion & Detection Assay offers a more versatile method for quantitating and imaging cells invading through an extracellular matrix (ECM) compared to a Boyden chamber device or membrane cell culture insert. Using Oris Cell Seeding Stoppers to create an invasion zone at the bottom of a microplate well, researchers can detect cell invasion in a 3-dimensional overlay of a Basement Membrane Extract (BME) component and monitor cell movement in real-time (Fig. 1, in the gallery on the right).

Features & Benefits of the Oris Cell Invasion & Detection Assay

Cell invasion assays have become a critical technique in screening compounds that can inhibit metastasis or promote angiogenesis1. True cell invasion is indicated by the ability of an adherent cell line such as HT-1080 human fibrosarcoma cells to use proteases and digest through an ECM component which can mimic an in vivo environment2,3.

The Oris Cell Invasion & Detection Assay allows researchers to study the specific mechanisms involved with cell invasion (Fig. 2) by making it easy to stain proteins associated with proteolysis and cytoskeletal structures in a single well. This cellular activity can be quantitated using a plate reader as well as imaged with a microscope or digital imaging system (Fig. 1).

Oris Cell Invasion & Detection Assay Protocol