Homepage > Knowledge DB > Technology info >

Find the best tools to study Mitochondria

A mitochondria is a multifunctional organelle, it is formed with an outer membrane, a highly invaginated inner membrane containing cristae, and a matrix. They are not only involved in energy production in a cell, but other additional cellular functions. Finding the role of mitochondria in cell physiology and pathology will help researchers to enhance the progress of modern medicine.

Use this interactive guide to easily select the right tool - click on the image

	Iron ion (Fe2+)	Calcium ion (Ca2+)	Lipophilic peroxide	Singlet oxygen	Superoxide
Reagent	Mito-FerroGreen	Rhod2-AM	MitoPeDPP	Si-DMA	MitoSOX
Principle	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence.	A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence.
Live Cell Staining	Yes	Yes	Yes	Yes	Yes
Staining Time	30 min	30-60 min	> 15 min	> 45 min	> 10 min
Ex / Em	505/535	553/576	452/470	644/670	510/590

	Mitophagy		Membrane Potential
Reagent	Mtphagy Dye	Keima-Red	JC-1	TMRM, TMRE
Principle	Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome.	A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome.	A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential.	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential.
Live Cell Staining	Yes	Yes	Yes	Yes
Staining Time	> 30 min	-	10-60 min	30-60 min
Ex / Em	530/700	440, 550/620	Monomer: 514 / 529 J-aggregation: 585/590	550 / 575

	Mitochondria Staining
Reagent	MitoBright LT series	MitoTracker series	DsRed	MitoRed	Rhodamine 123
Principle	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.	Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential.	A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent.	Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.	Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential.
Live Cell Staining	Yes	Yes	Yes	Yes	Yes
Staining Time	> 10 min	15-45 min	Expression in 8-12 hrs.	> 15 min	> 15 min
Ex / Em	493/508, 547/563, 643/663	490/516 ~ 644/665	558/583	560/580	507/529