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My accountFind the best tools to study Mitochondria
A mitochondria is a multifunctional organelle, it is formed with an outer membrane, a highly invaginated inner membrane containing cristae, and a matrix. They are not only involved in energy production in a cell, but other additional cellular functions. Finding the role of mitochondria in cell physiology and pathology will help researchers to enhance the progress of modern medicine.
Use this interactive guide to easily select the right tool - click on the image
| Iron ion (Fe2+) | Calcium ion (Ca2+) | Lipophilic peroxide | Singlet oxygen | Superoxide | |
| Reagent | Mito-FerroGreen | Rhod2-AM | MitoPeDPP | Si-DMA | MitoSOX |
| Principle | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with ferrous ion in mitochondria to emit green fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with calcium ion in mitochondria to emit red fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with lipophilic peroxides in mitochondria to emit fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and specifically reacts with singlet oxigen generated in mitochondria to emit red fluorescence. | A cell-permeant fluorescent probe that accumulates in mitochondria and reacts with superoxide generated in mitochondria to emit red fluorescence. |
| Live Cell Staining | Yes | Yes | Yes | Yes | Yes |
| Staining Time | 30 min | 30-60 min | > 15 min | > 45 min | > 10 min |
| Ex / Em | 505/535 | 553/576 | 452/470 | 644/670 | 510/590 |
| Mitophagy | Membrane Potential | |||
| Reagent | Mtphagy Dye | Keima-Red | JC-1 | TMRM, TMRE |
| Principle | Mtphagy Dye (included in Mitophagy Detection Kit) is a pH-sensitive fluorescent probe that accumulates in mitochondria and emits red fluorescence due to acidic conditions in a lysosome. | A pH-sentitive ratiometric fluorescent protein. The excitation spectrum changes accoring to pH. This protein shows high fluorescence ratio (Ex. 550 nm/440 nm) values in a lysozome. | A cell-permeant ratiometric fluorescent dye that accumulates in intact mitochondria due to the membrane potential. The excitation spectrum changes according to the mitochondria membrane potential. | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. Diffusion of the probes occurs in a damaged mitochondria that has the decreased membrane potential. |
| Live Cell Staining | Yes | Yes | Yes | Yes |
| Staining Time | > 30 min | - | 10-60 min | 30-60 min |
| Ex / Em | 530/700 | 440, 550/620 |
Monomer: 514 / 529 J-aggregation: 585/590 |
550 / 575 |
| Mitochondria Staining | |||||
| Reagent | MitoBright LT series | MitoTracker series | DsRed | MitoRed | Rhodamine 123 |
| Principle | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. | Cell-permeant fluorescent dyes that accumulate in intact mitochondria due to the membrane potential. | A red fluorescent protein that binds to intact mitochondria with the use of a transfection reagent. | Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. | Cell-permeant fluorescent dye that accumulates in intact mitochondria due to the membrane potential. |
| Live Cell Staining | Yes | Yes | Yes | Yes | Yes |
| Staining Time | > 10 min | 15-45 min | Expression in 8-12 hrs. | > 15 min | > 15 min |
| Ex / Em | 493/508, 547/563, 643/663 | 490/516 ~ 644/665 | 558/583 | 560/580 | 507/529 |
