tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences

Need an answer now?

Give us a call

Leave a message, we'll get back to you

VitroGel 3D Hydrogel ready-to-use hydrogel for 3D cell culture

See the related products

VitroGel™ 3D Hydrogel system for 3D cell culture

VitroGel™ 3D Hydrogel (TheWell Bioscience) is an animal origin-free hydrogel system closely mimicking the natural extracellular matrix (ECM) environment.
Cells grown in this hydrogel possess morphologies and cellular behaviors close to their natural state. 
cellphy tebu-bio: in vitro assays and laboratory services for more physiologically relevant experimental data

Therefore, VitroGel™ 3D fits perfectly with our "cellphy" offer aiming at providing life scientists with reagents and laboratory services mimicking the physiological environment of the Human body in vitro, for more physiologically relevant experimental data. Know more about cellply offer here.
2 VitroGel™ 3DFormulations available:
1. VitroGel™ 3D (cat. nr TWG001):

An animal origin-free hydrogel system mimicking the natural ECM: an ideal option for floating cell lines.

2. VitroGel™ 3D-RGD (cat. nr TWG002):

A "RGD" peptide modified hydrogel of VitroGel™ 3D promoting cell attachment and cell-matrix interactions for 3D-culture and 2D-coating (invasion/migration, chemiotaxis…). The best solution for adhesion cell lines.

VitroGel™ 3D - Principle & mechanism of action

VitroGel™ 3D is an animal origin-free polysaccharide hydrogel system with many advantages:
  • Ready-to-use: 
The hydrogel system is room temperature stable with neutral pH.
There is no additional reagents or preparation steps needed. Simply mixing the hydrogel solution with cell culture media for a ECM mimicking environment with 15-20 minutes!
After mixing for hydrogel formation, the system can be simply transferred from one container to another by pipetting. 
  • Transparent:
The hydrogel is transparent and compatible to different imaging systems for cell observation.
Each VitroGel™ 3D (10 mL/vial) is good for 2.5 96-well plates at standard mixing ratio. Downstream analysis can be done with standard protocols and reagents. No additional reagents or equipment are necessary.
  • Permeable:
Oxygen, nutrition and other molecules can easily move in/out the hydrogel system making this hydrogel very useful for drug discovery studies!
  • More accurate:
The morphologies and behaviors of cells grown in VitroGel™ 3D hydrogel system is similar to their natural state, which provide data to bridge in vitro and in vivo studies for many applications to obtain more physiologically relevant experimental data.
  • Convenient:
Cell harvesting is made easy. After 3D cell culture, cells can be easily harvested from the hydrogel by using standard centrifuging methods.
  • Injectable:
Using the right mixing ratio, the hydrogel becomes injectable for xenograft studies for example.
  • Easy-to-use with fast gelation:

Gelation starts immediately right after mixing and becomes stable in 15 min. Cells distribute homogeneously in the hydrogel.


How does TheWellBio's VitroGel Hydrogel works? tebu-bio

VitroGel™ 3D performances vs. other 3D cell cullture methods


VitroGel product performance vs. other 3D culture methods


Multiple applications on multiple cell types

The VitroGel™ 3D hydrogel system has great flexibilities of physical/chemical properties and the hydrogel preparation can also be manipulated to meet different applications.
VitroGel™ 3D is a powerful tool for your cell culture research:

VitroGel™ 3D can be used for 3D cell culture by simply mixing with cells. 

The hydrogel matrix supports cells to grow as 3D colonies over time.

Drugs or other chemical compounds can be added to penetrate into hydrogel system for screening.VitroGel Application in 3D cell culture and drug discovery

VitroGel in 3D cell culture

VitroGel™ 3D can be used to coat the substance that cells grow on.

The different stiffness and texture of the substance might lead to different cell behaviors. Also, as time goes on, cells might move into the hydrogel matrix for invasion/migration study.

VitroGel 2D application


By mixing the VitroGel™ 3D hydrogel solution and cell suspension (or other ionic solution) in a proper ratio (recommend 4:1 v/v), an injectable hydrogel can be formed and used for xenograft, drug delivery, bio-printing, microarray...
Additionally, the VitroGel™ 3D hydrogel properties and preparing protocols can be manipulated for different physical/chemical characteristic and gel formation times. The concentration of hydrogel solution can also be adjusted for different hydrogel strengths. Multiple applications can be done with these flexibilities.
VitroGel other applications
Customers have used this cell culture system for cancer and tumor cell biology, stem cells and tissue engineering, drug discovery and toxicity study and much more.
  VitroGel Applications tebu-bio


To know more and order your VitroGel™ 3D



VitroGel 3D & VitroGel 3D hydogel Ready-To-Use Hydrogel are available under Evaluation Sample Format (sufficient for 10 wells in 24-well plate; 2 mL), please contact your tebu-bio's local office.

See the related products