Good reasons to outsource your rec. protein production

• Choose to fully outsource your protein production project, or partially according to your specific needs
• Speed up your protein-based research program with ideal tools
• Move ahead with the most appropriate stategy, confirmed through initial feasibility studies
• Benefit from a fully customizable service platform for R&D-scale protein purification from E. coli or Hek293 EBNA cells (​production in CHO cells possible upon request: contact us for further information)

For many applications... which one is yours?

• Recombinant monoclonal antibody production
• Antigen production for antibody selection, biomarker assays or IVD
• Protein-Protein interaction studies
• Protein variants vs. protein partner screening
• Antibody screening
• Assay development for production/purification optimization
• Protein synthesis for development of functional assays
• Crystallography

How will your project evolve*?

1. Production optimization step: expression study and/or variant expression (multiplex format)

2. Protein production from small to large scale

3. Purification optimization steps: screening of different chromatography strategies and binding-elution conditions (multiplex purification)

4. Protein purification on Äkta pure system by:

• Classical methods (affinity, ion exchange and/or gel filtration chromatography)
• New innovative approaches (mixed mode and/or customizable affinity chromatography)

5. QCs (characterization) and delivery:

• ​Coomassie gel, Western Blot / ELISA
• Endotoxin level testing (FDA approved LAL test)
• Protein identification (MS, MALDI-TOF-TOF MS),
• Protein-protein interaction validation (GST-Pulldown assay, Binding studies and Enzyme kinetics by microcalorimetry (ITC)...)

Why you can trust tebu-bio for your protein production

• Process expertise
  Steps are customizable and allow for complete project outsourcing in an ISO9001-2008 and ISO14001:2004 certified quality management environment
• Complete transparency
  Project updates are provided weekly by your designated Project Manager, and your Customer Account Manager is always available for any other queries you may have
• No strings attached
  Process methods are made available to you for fast and efficient industrial exploitation with preservation of IP rights

* Example of steps for a standard project. All projects are fully customizable, so steps can be adapted according to your needs.

Image credits: PDB ID 3D90 (monomer and dimer), 5AUU, 4TPR