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3D cell culture spheroid production lab services

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3D cell cultures are artificial systems recreating the 3D environment seen in the body.

They enable complex in vitro cell/tissue interactions, thus mimicking in vivo environments.

Compared to 2D conventional cell culture, experimental data obtained with 3D cellular cultures are known to be more relevant and biologically accurate, closer to a “clinical context” (eg. predictive assays/preclinical therapeutic testing).

Organotypic spheroid production - tebu-bio's 100% tailored solution

tebu-bio’s 3D cell culture lab services consist of designing & producing healthy or diseased spheroid models from your own cell lines, and according to your requirements:

  • We start from your characterized cell line (intestine, kidney, pancreas, lung, liver…),
  • We generate organotypic spheroids at our premises, following experimental procedures designed according to your needs:
    • Scaffold-free hanging-drop, suspension, inclusion in specific 3D scaffolds (synthetic hydrogel (such as the animal origin-free VitroGel™ 3D hydrogel system, Collagen/Fibrinogen, Matrigel™), ultra low attachment (ULA) or magnetic systems
    • Porous bio-materials such as Alvetex® polystyrene (ReproCELL)...
  • We validate that the spheroid generation is optimal according to criteria and parameters of your choice (eg. morphology, viability, proliferation, survival, apoptosis…) 
Presented at #AACR18
Members of tebu-bio's lab team presented a poster "Time-lapse analysis of drug response and invasive capacity using patient-derived CRC microfluidic 3D cancer models under oxygen controlled conditions".
Download our poster covering a physiologically relevant study on 3D colon cancer models in microfluidic chips.
Download your copy here.

Downstream spheroid assay testing

Additional readouts can also be performed upon request for NME/compound spheroid-based testing.

  • Cytotoxic assays (eg. Drug & mitochondrial toxicity measurements...)
  • Migration & microfluidic-based assays (Oris (Platypus), Scivax (Scikon), ANFS (Nanosurface), 3D cell culture chip (Aim Biotech)…)
  • miRNA / mRNA profiling

  • Protein profiling & quantification
  • Signal transduction pathway profilingHCT-116 3D cell culture spheroid production by tebu-bio
  • Functional assays…

Image (right) - Our scientists used the HCT-116 cell line to product a batch of colon carcinoma spheroids. Cells were given the conditions to meet each other and form a cohesive and organized tissue. Spheroids capture epithelial tissue traits and respond to drugs and to 3D matrix environments in a close to in vivo manner. Image property of tebu-bio.


HCT-116 3D cell culture spheroids with paclitaxel by tebu-bio

Images (left) - Our scientists produced Colorectal carcinoma cell line HCT-116 spheroids which were treated with paclitaxel (top), and Liver carcinoma cell line HepG2 spheroids which were treated with staurosporine (bottom). The dose-dependent effect of paclitaxel and staurosporine on spheroid cell viability was determined with the CCK-8 colorimetric assay.


Our results are within the range of previously 3D cell culture spheroid production with staurosporine by tebu-biopublished results for paclitaxel (IC50= 4,03 and 9,5nM - Sirenko et al., 2015), and for staurosporine (IC50 =8,9±0,4 - Sirenko et al., 2015). Images property of tebu-bio.

Download the results of these PoC tests here - HCT-116 Spheroids with paclitaxel and HepG2 spheroids with staurosporine.


Why you can trust tebu-bio for your spheroid production

  • Process expertise
    Steps are customizable and allow for complete project outsourcing in an ISO9001-2015 and ISO14001:2015 certified quality management environment
  • Complete transparency
    Project updates are provided weekly by your designated Project Manager, and your Account Manager is always available for any other queries you may have
  • Lean and flexible project management
    From the Design of Experiment to the realization of the project, you control each of the stages of your projects. These "Go / No go" steps enable you to better control your expenses and time-schedule and bring flexibility in your R&D projects
  • No strings attached
    Process methods are made available to you for fast and efficient industrial exploitation with preservation of IP rights