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Mutant Nucleosomes for you advanced chromatin research

The nucleosome is the repeating unit of chromatin and serves as the physiological substrate for diverse chromatin interacting proteins. 

The core particle of the nucleosome is made up of a protein core of eight histone proteins (two copies each of the histones H2A, H2B, H3 and H4).

Mutations on Histone cause distinct changes in nucleosome structure, chromatin accessibility and gene expression, and are linked to numerous disease states.

The research has also revealed the importance of considering the entire nucleosome structure in chromatin interactions, as several of these mutations occur within the nucleosome core and modulate PTM crosstalk and multivalent binding mechanisms.

tebu-bio introduces to you a unique collection of Mutant Nucleosomes (Mutant Nucs) from the pioneer of the epigenetics research tools : Epicypher.

Acidic Patch Mutant Nucleosomes

The acidic patch is a negatively charged binding interface on the nucleosome surface formed by H2A and H2B residues. It is a central binding site for many nucleosome-associated complexes and frequently mutated in cancer.

Features & Applications

  • Homogenous, fully defined recombinant nucleosomes containing known acidic patch mutations (APMs)
  • Biotinylated and non-biotinylated versions available, enabling a variety of end-user applications
  • ONLY nucleosomes with acidic patch mutations available
  • Confirmed to contain negligible amounts of contaminating free DNA, which can interfere with downstream applications
  • Functional Q.C. testing to confirm mutations impact known acidic patch binding proteins

 

Decipher chromatin regulatory pathways Drug discovery pipelines Enzymatic assays
Protein interaction studies Structural studies Quantitative spike-in controls

Assess antibody performance

Useful negative controls for studies

utilizing acidic patch binding proteins & enzymes

Study the impact of acidic patch region

histone mutations on chromatin binding mechanisms

 

 

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Publications

• Kalashnikova AA et al. The role of the nucleosome acidic patch in modulating higher order chromatin structure. J R Soc Interface 10, 20121022 (2013). PubMed PMID: 23446052.

• Gamarra N et al. The nucleosomal acidic patch relieves auto-inhibition by the ISWI remodeler SNF2h. Elife 7, (2018). PubMed PMID: 29664398.

• Valencia AM et al. Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling. Cell 179, 1342-56 e23 (2019). PubMed PMID: 31759698.

• McGinty RK et al. Recognition of the nucleosome by chromatin factors and enzymes. Curr Opin Struct Biol 37, 54-61 (2016). PubMed PMID: 26764865.

• Skrajna A et al. Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding. Nucleic Acids Res 48(17), 9415-9432 (2020). PubMed PMID: 32658293

• Levendosky RF et al. Asymmetry between the two acidic patches dictates the direction of nucleosome sliding by the ISWI chromatin remodeler. ELife 8, (2019). PubMed PMID: 31094676