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New technology - Access the best iPSC-derived Human cells

There are a number of bottlenecks when it comes to research with human cells. Primary cells from donor tissue must come from reliable, yet sometimes limited sources, and their biology is highly variable. Tumor-derived cell line models are less representative of normal biology, and animal models may not always be an alternative.

Researchers need a reliable, scalable source of human cells

Thanks to a new, proprietary technology named opti-ox™, Bit Bio produces consistent, scalable, low cost, human iPSC derived cell types.

Applying the principles of computation to biology

opti-ox™ is a genetically engineered inducible TF expression system developed by BitBio, who, by implementing a high-throughput combinatorial approach, are able to decipher the optimal TF combinations for cellular reprogramming, demonstrated through in-vitro functional screens and validation.

Thanks to this innovative approch, you are able to obtain and work with high quality reprogrammed cells, ready-to-thaw and easy to use. They have been validated and published1, and can be used for HTS and functional screening purposes in many areas:

  • Basic research - normal and disease models, developmental and cell biology
  • Drug Discovery - Normal and Disease models, Drug screening, Target Discovery, Toxicology
  • Cell Therapy - Immune and other cell types, discovery programs...

Which cell types are already available?

This is a totally new approach, and Bit Bio's aim is to continuously extend their ioCELL portfolio, with new cell types in the pipeline.
 
You can already obtain the following products:
 
  • Ready for experiments as early as 2 days post revival
  • Ca. 88% glutamatergic neurons (VGLUT1 and VGLUT2 positive), 12% cholinergic neurons Day 11
  • Bulk RNA-seq analysis shows that the neurons have a rostral CNS identity
  • Exhibit neurite outgrowth, and form functional neural networks in 17 days that demonstrate drug response
  • Ready for experiments as soon as 2 days post-revival and contractility can be assessed as early as 3 days post-revival
  • Critical for metabolic studies, cells express GLUT4 by Day 7 post revival
  • Exhibit visible striated fibres and multinucleate myotube formation - Day 10
  • At day 10, post-revival skeletal myocytes are seen to contract in response to the muscle agonist, acetylcholine (50uM), with minimal cell detachment observed 

RT-qPCR analysis shows gene expression of key myogenic markers: MYH7 and MYH1, along with DESMIN, DYSTROPHIN, MYOGENIN, and TITIN

 

ioGlutamatergic Neurons

ioSkeletal Myocytes

Observe the rapid morphological changes from iPSCs upon induction of NGN2 expression using opti-ox cellular reprogramming. 7 day time course.

Following continued culture, ioMYOCYTES/skeletal can be seen to spontaneously contract in the dish at day 15 post revival, without any external stimuli.

Interested in learning more?


See how this innovative technology works and learn more about the available products
in these two posts on our blog:

 

References

1 - Molecular and functional characterization of stem cells-derived glutamatergic neurons (ioNEURONS/glut) in support of drug discovery applications
Poster / Charles River, Chesterford Research Park, Saffron Waldon, UK  - Contact your local tebu-bio office to obtain a copy of the poster