Fast & Ultrasensitive chromatin profiling technology - CUTANA™ CUT&RUN,CUT&Tag

Chromatin profiling assays enable you to map protein-DNA interactions that influence chromatin modelling and gene expression. 

If you’re looking for more sensitivity, faster protocols, and more reproducible results (and ideally starting from less starting cellular material), the novel immunotethering approaches for chromatin profiling CUT&RUN (Cleavage Under Targets and Release Using Nuclease)  and CUT&Tag (Cleavage Under Targets and Tagmentation) will be up to your expectations! 

CUTANA^TM CUT&RUN 

CUT&RUN uses a fusion of protein A, protein G and micrococcal nuclease (pAG-Mnase) to selectively cleave antibody-labelled chromatin. This strategy eliminates immunoprecipitation steps, greatly simplifying your assay workflow. Clipped chromatin fragments are isolated from solution and used for NGS.

• Robust Assays

Diverse sample inputs and targets

• Fewer cell input required:

Protocols are optimized for 500K

• Obtain higher sensitivity

Improved signal-to-noise compared to ChIP-seq 

• Simplify your workflow

Eliminate the chromatin fragmentation step of ChIP-seq experiments

CUTANA^TM CUT&Tag 

CUT & Tag is based on the same technology used in CUT&RUN, which catalyzes simultaneous cleavage and sequencing adapter ligation at antibody-bound chromatin through an innovative fusion of protein A, protein G and Tn5 transposase.

•  Access to ultra-low / single-cell inputs

Generates quality data down to 1,000 cells without adjusting the workflow 

•  No library prep - save money 

• Obtain higher sensitivity

Improved signal-to-noise and genomic coverage compared to ChIP-seq  

• Simplify your workflow

The entire workflow can be performed in a single tube 

Discover CUTANA CUT&RUN in detail in
this post on our blog