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SARS-CoV-2 - RT-qPCR to detect viral RNA

With the outbreak of SARS-CoV-2, RT-qPCR has become an important means of virus detection. RT-PCR is a technique which makes it possible to carry out a PCR (polymerase chain reaction) from an RNA sample. In scientific research, the RT-qPCR technique, for real-time quantitative PCR, is one of the most widely-used methods for quantifying a type of RNA initially present in a sample, due to its sensitivity, speed, and relatively low cost.

As a distributor of specialty reagents and physiologically relevant in vitro assays, we are actively in contact with our manufacturers to bring you their validated solutions for virus detection. The following kits, assays and controls are of particular interest.

RT-qPCR kits and assays for SARS-Cov-2 viral RNA detection

Mag-Bind® Viral RNA Xpress kit

The Mag-Bind® Viral RNA Xpress Kit follows a magnetic bead-based approach for rapid and reliable isolation of viral RNA from nasopharyngeal (NP) swab specimens that are dry or in viral transport media (VTM).

China and US CDC RT-PCR detection assays for SARS-CoV-2

This assay has successfully detected SARS-CoV-2 in positive control samples N gene and human RNAse P (RNP) gene to assess the specimen quality. It’s based on CDC protocol, ISO 13485 standards of manufacturing reagents.

The technology is TaqMan™ Real-Time RT-PCR with FAM probes for higher specificity. It targets 3 sequences of SARS-COV-2 N gene (NC_045512.2) and it also includes a positive control.

SARS-CoV-2 Real Time RT-PCR Detection Assay - 100 reactions for only 598 Euros

BlazeTaq™ SYBR Green One-Step RT-qPCR Kit

This kit is based on quantitative reverse transcription PCR (RT-qPCR) that uses RNA as the starting material. It offers a convenient master mix to convert RNA to DNA and quantify in a one-step reaction. The kit is supplied with reverse transcriptase and a 5X master mix with a hot-start Taq DNA polymerase, dNTP and all required buffer components. BlazeTaq™ One-Step SYBR Green RT-qPCR kit is compatible with diverse real-time PCR instruments. Download the User Manual here to learn more.

  • High sensitivity- accurate detection of target RNA from as little as 0.1 pg RNA extract
  • High specificity, with the minimal level of primer-dimer and non-specific product formation
  • High amplification efficiency over wide GC-content range.

Probe/Primer Synthesis for SARS-COV-2 Detection

In alignment with the official website documents of the WHO and the Chinese & USA CDC, we can offer a series of primers available for COVID-19 detection in batches.

Viral RNA positive controls

We provide a range of RNA controls which enable you to:

  • Control the reverse transcription efficiency
  • Achieve unification of standards
  • Reduce false negative rates
  • Make the detection results more reliable
  • Greatly improve the accuracy of the kit detection degree

GLuc-ON™ promoter reporter clones - CCL2 and ACE2

Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of human and  mouse promoters using live cell assays

Currently we offer both single-reporter and dual-reporter vector systems. The single-reporter system uses GLuc, mCherry, or GFP as the promoter reporter. The dual-reporter system uses GLuc as the promoter reporter and SEAP (secreted Alkaline Phosphatase) as the internal control for signal normalization.

I-Yin Chen et al (doi:10.1128/JVI.02560-09) revealed high increase of the human gene CCL2 during coronavirus infection, making it a good candidate for promoter reporter of the interaction of the Spike protein with ACE2. A transient transfection of the dual construction would be an efficient cell-based assay to screen inhibitors of Spike-ACE2 interaction.

Gene synthesis and mRNA production for therapeutics perspective or in vitro diagnostic assay development

We offer gene synthesis and mRNA for the ORFs of the SARS-CoV-2.

  • For rapid vaccine development
  • To produce the SARS-Cov2 protein in HEK or CHO
  • To express the proteins in targeted cells

There are catalogue mRNAs:

  • Optimization of the mRNA delivery, eGFP, Fluc, …
  • CAS9 mRNA combined with the Smart Aliquotor for rapid KO to decipher the role of endogenous genes in SARS-Cov2 infection, for example ACE2 receptor KO

In both cases, electroporated denditric cells and in direct injection mRNA can induce immune responses against tumour antigens. Notably, a pulse with ovalbumin(OVA)-encoding mRNA can boost a tumour-reducing immune response (Pardi, N., Hogan, M., Porter, F. et al. mRNA vaccines — a new era in vaccinology. Nat Rev Drug Discov 17, 261–279 (2018). doi.org/10.1038/nrd.2017.243)

We can also produce anti-sense oligonucleotides and aptamers including long RNA ones (100nt).