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Mag-Bind® Viral DNA/RNA 96 Kit

For high throughput total nucleic acid isolation from bodily fluids

The Mag-Bind® Viral DNA/RNA 96 Kit (cat. Nr M6246) is for fast and high throughput viral RNA and DNA isolations from numerous types of biological samples.

  • Unique Omega Bio-Tek Mag-Bind® magnetic bead technology
  • High quality viral nucleic acids obtained free of proteins, nucleases, and other impurities
  • Fully automatable and ready-to-load scripts on the KingFisher™, Tecan, and Hamilton platforms
  • Dedicated technical and application support to expedite setup and validation time
  • Versatile procedure adaptable to most automated systems and scalable up or down depending on the amount of starting sample
  • Ideal for sera, plasma, saliva samples (see user manual)
  • Compatible with NP swabs, aspirates and BAL samples (see supplementary protocol)
  • Purified nucleic acids ready for direct use in downstream applications, such as amplification or other enzymatic reactions.

▶︎ Contact us to discuss how the MagBind kit can work for you

 

 

Viruses already detected using Omega Bio-Tek's Nucleic Acid Viral kits
2019 n-COV (SARS-CoV-2; COVID-19) Infectious Bronchitis virus
2012 MERS-CoV Influenza A virus subtype H1N1 (A/H1N1)
Arboviruses Influenza A
Avian Encephalomyelitis Virus Influenza B
Avian leukosis virus subgroup J Insect-specific flaviviruses, mononegaviruses, and totiviruses
Bovine Viral Diarrhea Virus (BVDV) Marek's disease virus
Canine distemper virus Murine norovirus 1
Coxsackievirus A6 Orf virus (ORFV)
Coxsackievirus B3 Porcine circovirus type 2 (PCV2)
Crimean-Congo hemorrhagic fever virus Porcine reproductive and respiratory syndrome Virus (PRRSV)
Dengue virus Rabies virus
GB virus C Rotavirus
Hepatitis A virus types 1 and 3 Sheep pox virus
Hepatitis B virus SIV
Hepatitis E West Nile virus
HIV Zika virus (ZIKAV)

 

50 µL of ZeptoMetrix’s NATtrol Influenza A/B Positive Control standard was spiked into 150 µL of human serum. Viral nucleic acids were extracted using Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit. Average Ct values obtained after amplification using Influenza B primers are shown above. The results indicate positive detection of Influenza B in both undiluted and 10-fold diluted purified samples. HBV virus (in quantities of 10 and 1 infectious unit[s]) was spiked into 200 µL of human serum. Viral nucleic acid was isolated with Omega Bio-tek’s Mag-Bind® Viral DNA/RNA 96 Kit and a comparable kit from Company A according to manufacturer’s recommended protocols. 5 µL of template was used for a SYBR® Green labeled qPCR reaction, which was replicated 4 times. The resulting mean Ct values are shown in the above figure. Nucleic acid was isolated from 200 µL of human whole blood with Omega Biotek’s Mag-Bind® Viral DNA/RNA 96 Kit and a kit from Company A using the manufacturer’s recommended protocols. The extractions were eluted in 100 µL. 3 concentrations of template were used as templates in a SYBR® Green labeled qPCR reaction. Each reaction was performed in quadruplicate and the mean Ct value is depicted in the above figure.

Proven capacities with numerous publications - most recently:

  • Fauver J.R. et al  “A Reverse-Transcription/RNase H Based Protocol for Depletion of Mosquito Ribosomal RNA Facilitates Viral Intrahost Evolution Analysis, Transcriptomics and Pathogen Discovery” (2018) Virology, doi:10.1016/j.virol.2018.12.020.
  • Gu S.P. et al. “Identification and Phylogenetic Analysis of the Sheep Pox Virus Shanxi Isolate” (2018) Biomedical Research, doi:10.4066/biomedicalresearch.29-17-431.
  • Fauver J.R. et al. “Xenosurveillance Reflects Traditional Sampling Techniques for the Identification of Human Pathogens: A Comparative Study in West Africa” (2018) PLOS Neglected Tropical Diseases, doi:10.1371/journal.pntd.0006348.‌
  • Ravi P. et al. “Detection of Arboviruses in Blood and Mosquito Slurry Samples Using Polymer Microchip” IEEE Xplore, doi:10.1109/HIC.2017.8227611.‌
  • Gloria-Soria A., et al. “Infection Rate of Aedes Aegyptimosquitoes with Dengue Virus Depends on the Interaction between Temperature and Mosquito Genotype.” Proceedings of the Royal Society B: Biological Sciences (2017), doi:10.1098/rspb.2017.1506.