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Get 20% off DyLight Fluo Secondaries

Get 20% discount to try Rockland high performance near-IR DyLight™ secondaries, whether you wish to optimize the specificity and signal of your Multiplex Western blot, IP/Western Blot or Immunofluorescence assays. Their matching fluorescent western blot buffer MB-070-050TF is also included in the offer to ensure optimal performance!

Low background / High signal Multiplex Western Blot 

Fluorescence imaging systems allow detection of your proteins in western blot, providing a longer dynamic range read-out compared to chemiluminescence. This allows to detect both low and high concentrated proteins in the same assay. Moreover the multi-colour capacity of the readers allows precise comparison of 2 proteins at a time. A perfect combination is offered by the use of DyLight 680 and 800 on the LI-COR Odyssey near infrared device and any other conventional instrumentation. When combined with matching blocking buffer (MB-070-050TF), Rockland DyLight™ near-IR Dyes secondaries ensure two-colour Western Blot detection with low background and high signal.  

 

 

Anti-tubulin was detected using a DyLight™ 680 conjugate. Anti-TNFa was detected using a DyLight™ 800 conjugate.The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.

Check our most used secondaries in the table below, all part of the offer.
Pre-Adsorbed references are recommended for optimal results.

 

DL680 conjugate

DL800 conjugate

Secondary antibody

Not Pre-Adsorbed

Pre-Adsorbed

Not Pre-Adsorbed

Pre-Adsorbed

Anti-RABBIT IgG (H&L) (GOAT) Antibody

611-144-002

611-144-122

611-144-002

611-145-122

Anti-MOUSE IgG (H&L) (GOAT) Antibody

610-144-002

610-144-121

610-145-002

610-145-121

Anti-HUMAN IgG (H&L) (GOAT) Antibody

609-144-002

609-144-123

609-145-002

609-145-123

Anti-RAT IgG (H&L) (GOAT) Antibody

612-144-002

612-144-120

612-145-002

612-145-120

 

 

 

Immunofluorescence microscopy of a-tubulin in A431 cells using DyLight™ 680-conjugated Fluorescent TrueBlot® anti-mouse IgG for detection. (a) Merged a-tubulin (red)/DAPI (blue) image shown. (b) secondary antibody only.

Benefit from the specificity of monoclonal TrueBlot secondaries in immunofluorescence application with the Dylight TrueBLot formats.

Most popular Anti-Rabbit and Anti-Mouse Fluorescent TrueBlot® included in the offer

 

DL680 conjugate

DL800 conjugate

Secondary antibody

Monoclonal

Monoclonal

Fluorescent TrueBlot®: Anti-Rabbit IgG

18-4416-32

18-4516-32

Fluorescent TrueBlot®: Anti-Mouse Ig

18-4417-32

18-4517-32