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High Quality Secondary Antibodies

Secondary antibodies bind to the primary antibody to assist in detection, sorting and purification of target antigens. To enable detection, the secondary antibody must have specificity for the antibody species and isotype of the primary antibody being used and generally is conjugated. Select a secondary antibody that recognizes the host species used to produce your primary antibody of interest, i.e. choose an anti-mouse secondary antibody to detect a mouse monoclonal primary antibody. 

However, identifying the optimal secondary antibody requires knowledge of the detection assay.   For example: Western blot and ELISA can be performed using colorimetric, chemiluminescent, and fluorescence reporter systems, while immunofluorescence and flow cytometry are generally limited to fluorescent reporter labels. In all of these instances, a conjugated secondary antibody is required.

Special 25% discount off Rockland Inc.'s secondary antibodies!

Simply mention promo code DIST-JAN25SEC on your order to benefit from this offer, valid up to February 28th, 2018*.

Download your guide to a selection of Rockland secondary antibodies and other products here
Secondary antibody - Goat IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed - Rockland and tebu-bio Secondary antibody - Rat IgG (H&L) Antibody Pre-Adsorbed - Rockland at tebu-bio Secondary antibody - 601-1902 - Bovine IgG (H&L) Antibody Texas Red™ Conjugated - Rockland at tebu-bio
ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) (p/n 612-1125). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Human Serum Proteins) (p/n 612-1125) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher. Western Blot of Goat anti-Bovine IgG Texas Red Conjugated Secondary Antibody. Lane 1: Bovine IgG. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Bovine IgG. Other band(s): Bovine IgG splice variants and isoforms.
 
 
 
*Offer non-cumulative with any other special offers, discounts or trade agreements. For further information, contact your local tebu-bio office.