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Cell Counting Kit 8 (CCK-8) - An easier way for MTT assay users to analyse cell viability on 2D or 3D cell culture

Test our new and more sensitive colorimetric cell viability assay : Cell Counting Kit 8 (CCK-8)

Based on the use of a highly water-soluble tetrazolium salt (WST-8), the Cell Counting Kit-8 (CCK-8) from Dojindo Laboratories, allows a better and easier colorimetric assays for the determination of cell viability, cell proliferation and citotoxicity assay in comparison to the classical MTT cell viability assay

The 5 main benefits (to keep it short!)

  • Viabilitiy analysis on classical or organoid cell culture
  • Re-use of cells after colorimetric measurement
  • Highest sensitivity
  • Less cellular toxicity
  • Easier and quicker - One ready-to-use bottle / no need to dissolve Salt / no need to lyse cells

Thanks to its water-soluble salt (WST-8), the CCK-8 colorimetric assay is currently the most well accepted assay in academia,
in comparison with other Tetrazolium salt assays.

Interested in trying one of these assays for free?

We have a limited number of samples available for you to try the CCK-8 kit in your lab.

Yes, please send me a free sample of the CCK-8 kit

Offer valid while stocks last, based on a first-come first-served basis. Simply place your request via the link above.

See how CCK-8 was used with Spheroids in our lab

CCK-8 allows determination of cell toxicity in a much easier and quicker way than microscopy scoring.
Dora Sabino, Phd, is one of our scientists at our labs based in France. She is our 3D cell culture spheroid production specialist, and shares this feedback on CCK-8 after using it in one of her projects:

"When your model of choice is a 3D culture, the colorimetric CCK-8 represents a significant gain of time relative to microscopy scoring methods for accessing viability, proliferation or cytotoxicity.

Incubation of HCT-116 and HepG2 spheroids with WST-8 (CCK-8 cell counting kit) for as little as two hours enables reliable measurement of absorbance and the determination of IC50 values and cytotoxicity caused by paclitaxel and staurosporine.

The CCK-8 kit is an invaluable tool for anyone interested in spheroid-based studies."

Download the PDF with more information & Dora's results here


Learn more about WST-8 and our Cell Counting Kit-8 (CCK8) on Being bioreactive...
Watch the videos!