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Cell Counting Kit 8 (CCK-8) - An easier way for MTT assay users to analyse cell viability on 2D or 3D cell culture

New and more sensitive colorimetric cell viability assay : Cell Counting Kit 8 (CCK-8)

Based on the use of a highly water-soluble tetrazolium salt (WST-8), the Cell Counting Kit-8 (CCK-8) from Dojindo Laboratories, allows a better and easier colorimetric assays for the determination of cell viability, cell proliferation and citotoxicity assay in comparison to the classical MTT cell viability assay

The main benefits

  • Reliable and sensitive assay for classical or 3D cell culture spheroid viability analysis (drug testing)
  • Easy assay - no need for preparation: One ready-to-use bottle – one year storage at 4°C
  • No need to lyse the cells for the colorimetric measurement of cellular viability
    ⇒Cells can be re-used after measurement
  • Weak cellular toxicity

Thanks to its water-soluble salt (WST-8), the CCK-8 colorimetric assay is currently the most well accepted assay in academia, in comparison with other Tetrazolium salt assays.

New or returning customer? Get a special offer and discounts in September

Get in touch with your local tebu-bio office to discover which special offer or discount you can benefit from when you order a CCK-8 kit this month.


Interested in trying one of these assays for free?

We have a limited number of samples available for you to try the CCK-8 kit in your lab.

Yes, please send me a free sample of the CCK-8 kit

Offer valid while stocks last, based on a first-come first-served basis. Simply place your request via the link above.

See how CCK-8 was used with Spheroids in our lab

CCK-8 allows determination of cell toxicity in a much easier and quicker way than microscopy scoring.
Dora Sabino, Phd, is one of our scientists at our labs based in France. She is our 3D cell culture spheroid production specialist, and shares this feedback on CCK-8 after using it in one of her projects:

"When your model of choice is a 3D culture, the colorimetric CCK-8 represents a significant gain of time relative to microscopy scoring methods for accessing viability, proliferation or cytotoxicity.

Incubation of HCT-116 and HepG2 spheroids with WST-8 (CCK-8 cell counting kit) for as little as two hours enables reliable measurement of absorbance and the determination of IC50 values and cytotoxicity caused by paclitaxel and staurosporine.

The CCK-8 kit is an invaluable tool for anyone interested in spheroid-based studies."

Download the PDF with more information & Dora's results here

Watch the videos...

...and learn more about WST-8 and our Cell Counting Kit-8 (CCK8) on
our scientific blog Being bioreactive