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Cell Sourcing & Signalling Innovation

May 2015

News Highlight

Factors affecting human islet cells quality

Islets are widely used for transplantation to restore beta cell function from diabetes, offering an alternative to a complete pancreas transplantation or an artificial pancreas. Human Islets for Research (HIR)™ are obtained in a proprietary process of pancreas digestion and islet purification that results in uniformly high quality HIR™ for diabetes investigators.

Since we are not involved in clinical transplantation, all of our islets processed are available for research, rather than only a portion that may become available as not meeting clinical requirements.

Cardiac cellular models

Jean-François invites you to take a look at several cell types which can be used as models for studying different aspects of cardiovascular functions and diseases.

He also reviews some recently published results highlighting the importance of securing your primary cell sourcing.

Product Focus

Enriched Human Tregs

We are proud to announce the development of enriched T regulatory cells. For those of you who know your cell surface molecules, you may be glad to read that this enriched product has over 2 million viable CD4+CD3+CD45+ T cells per vial. The Tregs are CD25+, CD127 dim, and FoxP3+.

Adherent cell migration without cell culture inserts

Wound healing assays are normally performed using a scratch assay, which has high variability. The unique Oris™ Cell Migration Assay plate design generates highly reproducible results using a microscope, digital imaging system or fluorescence plate reader. Simply adhere the seeded cells in each well, remove the stopper to create a detection zone, and measure the migration.

The Oris™ Cell Migration Assay is an ideal alternative to the scratch assay as the cell seeding stoppers create a consistent detection zone to monitor 2-D closure.

Technical Corner

Imaging techniques compatible with 3D culture of cells grown in Alvetex Scaffold

As scientists better understand the benefits growing cells in 3D and routinely adopt 3D culture techniques, methods for visualising cells must also be adapted and optimised.
To visualise cells cultured in Alvetex®Scaffold, a range of appropriate methods are available. These are discussed in “A Review of Imaging Techniques Compatible withThree Dimensional Culture of Cells Grown in Alvetex®Scaffold”...


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