Acting and reacting in Life Sciences and Biotechnologies

Acting and reacting in life sciences and biotechnologies

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NEWS FLASH

JULY 25, 2017

Acting and reacting in Life Sciences and Biotechnologies

What is "being bio-reactive"? We define it as opening new doors, exploring new areas, staying informed aboutbreaking news & developments in Life Sciences and the fast evolving world of biology.

Here's a selection of information & events from our blog this week...

Cell-based assays with hPBMCs for cell therapy and drug discovery

25/07/2017 BY PHILIPPE FIXE, PhD

Human Peripheral Blood Mononuclear Cells (hPBMCs) are essential for designing cellular models to be used in cell therapy and drug discovery research programs [1-7, 12]. Being able to access reliable and ethical sources of well-characterized hPBMCs is now becoming fundamental for running physiologically relevant cell-based assays. Why use Human Peripheral Blood Mononuclear Cells (hPBMCs)? While cell lines are an excellent tool for early phase discovery and screening, human primary cells... [ Read more ]

Cell-based assays with hPBMCs for cell therapy and drug discovery

cAMP signals visualized in live human pancreatic primary cells

21/07/2017 BY PHILIPPE FIXE, PhD

DIn a recent publication, researchers from the University of Miami Miller School of Medicine (USA) describe that Serotonin released by human beta cell inhibits glucagon secretion by alpha cells. They demonstrated that this paracrine loop was mediated via the cAMP pathway. To do so, they captured in live human pancreatic islet cells cAMP signals using a specific fluorescent biosensor.... [ Read more ]

cAMP signals visualized in live human pancreatic primary cells

Microcell arrays obtain more accurate data from single living cells

17/07/2017 BY FREDERIC SAMAZAN

Recent improvements in our abilities to perform fluorescence microscopy on primary cells, coupled with advances in pipelines for quantifying and extracting data, have made possible a better understanding of the temporal complexity of cell signalling pathway. However as living cells don't stay still, classical imaging and studying method present some drawbacks for single cell analysis and tracking such as... [ Read more ]

Analysis tool for direct and sandwich ELISA - convenient and reliable

11/07/2017 BY DIMITRI SZYMCZAK

Analyte quantification (in pg/ml or ng/ml) such as IL-6, TNFalpha and numerous other proteins is conveniently performed with immunoassays called ELISA kits. At tebu-bio, we not only provide more than 10 000 ELISA kits but we also support researchers through our techserv team who are on-hand to suggest the best kits and to answer any questions. Being part of that team, I have noticed a number of often asked questions that can be resumed in one : What is the best method for data analysis?... [ Read more ]

Analysis tool for direct and sandwich ELISA - convenient and reliable

Ras-Raf-MEK-ERK Pathway: a broad range of research tools

07/07/2017 BY ISABELLE NOBIRON

The Ras-Raf-MEK-ERK Pathway is an important cell signaling route with many implications for cancer biology and therapeutic development. Dis-regulation of some of this pathway's proteins expression and phosphorylation status are observed in about one-third of all human cancers. Access to specific tools to study this pathway is essential to a better understanding of its role in cancer for novel drug development. If you are working on this topic, you'll be interested in taking a look at the range of reagents on offer for a wide variety of applications.... [ Read more ]

Ras-Raf-MEK-ERK Pathway: a broad range of research tools

Optimizing the CAS9 messenger RNA for vector-free gene editing

28/06/2017 BY DIMITRI SZYMCZAK

For transgenesis and therapeutic perspectives, vector-free delivery of the CAS9 for gene editing is highly preferable. It avoids the risk of hitting the genome due to the vector integration. Directly using CAS9 mRNA is a top option. Furthermore, as a new promising class of therapeutic biologics, messenger RNA are the subject of numerous studies. This year, Matthew Porteus's and Trilink's teams revealed key and practical results at the ASGC with a poster you can download ... [ Read more ]

Optimizing the CAS9 messenger RNA for vector-free gene editing