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Fluorogenic HDAC Substrate

Assay Protocol:


Assay buffer ( BPS catalog number 50031); Assay developer (BPS catalog number 50030); HDAC Substrate (catalog number 50037)

Step 1:

Adding all reaction mixture to a low binding 0U0C black plate (VWR catalog number 62408-936)
2 μl of HDAC2 (0.05 μg/μl)
38 μl of HDAC assay buffer (BPS catalog number 50031)
5 μl of 1 mg/ml BSA
5 μl of 200 μM substrate (BPS catalog number 50038)
Incubate at 37 ºC for 30 min.

Step 2:

Stop the reaction
Add 50 μl of HDAC assay developer (2x) (BPS catalog number
50030) and incubate the plate at room temperature for 15 min

Step 3:

Read samples in a microtiter-plate reading fluorimeter capable of excitation at 485 nm and emission at 528 nm.


Edition 13.01.2010 – please refer to documentation provided with the substrate to implement
the assay