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StemBeads FGF2 FAQs

While using StemBeads, my growth medium is more yellow than usual. Is that a problem?

Because you are changing media less frequently, the media pH will change quicker sometimes indicated by yellow media. While this does occur, the cells growing are unaffected, with no effects on cell growth and/or differentiation. Try plating cells at a lower density to avoid such yellow media. StemBeads FGF2 work optimally at medium to lower densities as cells expand rapidly.

Towards the end of my culture, I notice some StemBeads stuck to cells or colonies. Is that a problem?

StemBeads release the majority of FGF2 by 7 days, so if you do see residual StemBeads in your dish by the time you split, they most likely are not active. The StemBeads are biodegradable, and with time will disappear and not harm the cells.

How much time will I save with StemBeads FGF2?

Instead of changing media daily for pluripotent cells, now you can change media twice in one week. That saves over 65% of your valuable time!

What is the shelf life of StemBeads FGF2?

StemBeads can be stored at 4°C for 6 months without loss of activity.

What cell types can I grow using StemBeads FGF2?

As of now, we have extensive testing on human pluripotent stem cells (ESC, iPSC) and mouse and human neural stem cells. However, we are confident that StemBeads FGF2 will work with any FGF2 dependent cell type such as MSCs, and other cell lines.

What concentration does StemBeads FGF2 release at?

The StemBeads are designed to release at 10 ng/ml.