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Petaka FAQ

1. How big is a Petaka™ cell culture bioreactor?

A PetakaTM G3 plate footprint is the same size as a microtitre plate, but it is thinner. The outer dimensions are about 127mm long x 85mm wide x 5mm thick.

2. What material is used for the Petaka™ bioreactor’s wall for growing cells?

The PetakaTM G3 LOT bioreactor has two growth surfaces made from a corona treated crystal polystyrene. The PetakaTM G3 HOT is made from a corona treated co-polymer with a gas diffusion rate 3 times higher than crystal polystyrene. Each growth surface has a thickness of about 1mm. The two surfaces are about 3mm apart.

3. What are the optical properties of a Petaka™ bioreactor?

The PetakaTM G3 bioreactor’s surfaces have a refraction index of 1.57. The light absorption spectrum is from 310nm to 670nm absorbance.

4. How does the Petaka™ bioreactor foster cell growth?

Cell growth is optimized by providing adequate surface energy (Dynes) on the cell growth surfaces and by maintaining a balanced amount of oxygen and carbon dioxide dissolved in the media.

5. How much dehydration occurs over time if I preload my Petaka™ bioreactor with media?

Media introduced in a PetakaTM G3 LOT has a negligible dehydration rate. Spontaneous dehydration occurs at the following rates:

 

 

 

 

 

 

 

 

 

6. What is the filter material?

The filter is made of a polytetrafluoro-ethylene combined with 100% polypropylene support components. All materials conform to FDA requirements. The filters are 100% integrity tested and lot coded.

7. How does the Petaka™ bioreactor allow for cell dormancy?

The PetakaTM G3 bioreactor isolates the cell culture growing inside the chamber from environmental conditions, such as ambient air and moisture, therefore avoiding oxygen access and slowing pH changes.

8. Does the Petaka™ bioreactor require carbon dioxide for incubation?

No, the PetakaTM G3 cell culture bioreactor does not require a supplemented carbon dioxide atmosphere.

9. Do I need an incubator when using the Petaka™ bioreactor to culture cells?

Yes. PetakaTM G3 cell culture bioreactor can culture cells in any type of standard incubator, whether dry or humid, and with or without controlled CO2, just as long as temperature control is provided for temperature stability.

10. What kinds of cells yields should I expect when culturing cells in a Petaka™ bioreactor?

The PetakaTM G3 cell culture bioreactor has 150cm2 of surface area available for cell attachment and a 24ml capacity for media and growth factors. The yield depends on the cell type, but is approximately 200,000 cells per cm2, or roughly 30 million cells per bioreactor. The experimental average yield is about 750,000 cells per ml of media.

11. How long can cells remain dormant in a Petaka™ bioreactor?

Cells can remain dormant inside the PetakaTM G3 from between 7 to 200 days. Durations of longer periods, depending on cell type, initial pH and maintenance of temperature stability during the dormancy period, are possible. A 50% viability rate has been achieved from dormant cultures.

12. Is the Petaka™ bioreactor reusable?

For a given cell culture, the media may be exchanged several times inside the PetakaTM G3. PetakaTM G3 may also be reseeded with the same cell line to save using a new bioreactor. However, PetakaTM G3 is not autoclavable and should not be reused other than this. After use, PetakaTM G3 should be discarded. Discarded PetakaTM G3 bioreactors are made of harmless plastic and may be recycled.

13. What will happen to the cells in a Petaka™ bioreactor if it is dropped on a hard surface?

While the PetakaTM G3 itself is very strong and robust, if the bioreactor were to be dropped accidentally on a hard surface, some cells will detach from the growth surface. After a few minutes of incubation, these cells normally reattach without complications.

14. Can I use the Petaka™ bioreactor to obtain cell bioproducts, like proteins or antibodies?

Yes, particularly with cell culture methods for all kinds of anchored cells, such as CHO cells. However, with suspended cells, such as hybridomas, that need high concentrations of O2 in the medium; the PetakaTM G3 HOT would be more suitable.

15. Can the contents of my Petaka™ bioreactor be contaminated by outside environmental conditions, such as bacteria or viruses?

While highly unlikely, the PetakaTM G3 bioreactor’s contents can become contaminated. There is a risk, if using a contaminated injection tip or if the PetakaTM G3 septum surface is contaminated, when introducing cells into the PetakaTM G3 through the port access. To reduce the introduction of contaminants to the PetakaTM G3 interior chamber, we recommend disinfecting the port surface before tip penetration with 90% ethanol and a suitable flame.