tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences
X

Need an answer now?

Give us a call

Leave a message, we'll get back to you





Viral Load Control FAQ

What is the intended use for Quantitated Viral Load Controls?

Our Quantitated Viral Load Controls are intended for determining viral load in unknown samples and for use as a control to validate, monitor and troubleshoot nucleic acid extraction procedures.

What is the composition of Quantitated Viral Load Controls?

This product contains purified, whole virus particles suspended in TNE with 0.1% Sodium Azide at a known concentration of 1.5x107 virus particles (vp)/ml.

How is the viral particle concentration determined?

Virus particle concentration is determined by a proprietary procedure developed at ABI.

How are Quantitated Viral Load Controls used?

Our Quantitated Viral Load Control should be extracted and amplified alongside unknown samples.

Do you provide a DNA copy number for Quantitated Viral Load Controls after extraction and amplification?

DNA copy number can vary depending on the diluent and extraction/ amplification method used. Therefore, wer do not provide a DNA copy number for Quantitated Viral Load Controls after extraction and amplification. We provide a known concentration of virus particles suspended in a buffer appropriate for further dilution in a diluent of your choice, to help determine extraction efficiency of your procedure and/or viral load of your unknown samples.

Do you provide a conversion factor to the WHO (World Heath Organization) International Standards?

We do not provide a conversion factor to the WHO International Standard. The conversion factor to the International Units is specific to the extraction/ amplification method and the diluent used by the customer, and therefore, should be determined by the customer.

How are Quantitated Viral Load Controls supplied?

They are offered in multiples of five single use vials. Each vial contains 175µl of Viral Load Control at 1.5x107 virus particles (vp)/ml.

What is the difference between your Quantitated PCR Controls and Viral Load Controls?

Our Quantitated Viral PCR Controls contain purified DNA and are used for preparing standard curves for quantitated PCR assays. They are also intended to be used as a positive control in nucleic acid amplification reactions, in both standard and real time PCR.

Our Quantitated Viral Load Controls contain purified, whole virus particles. They are intended to be used along side unknown samples in DNA extraction and amplification procedures, for viral load determination of unknown samples and for use as a control to monitor and validate nucleic acid extraction procedures.