SilenciX^® cells are Tebubio's ready-to-use, knockdown (KD) cell lines using a unique siRNA Delivery system.

The SilenciX^® technology combines RNAi technology, EBV derived vectors and a Designer of Small Interfering RNA (DSIR) program.

This enables the generation of syngenic, ready-to-use and stable cellular in vitro models stably silenced for a gene of interest.

This technology has already been validated in the scientific literature in various biological domains and applications such as DNA Repair, Epigenetics, Ubiquitination and the cell cycle, Drug discovery, cell signalling and mechanism of action.

The SilenciX^® technology has already been shown to be compatible with numerous mammalian cell types including Human, Primate and Rodent cells.

Why choose SilenciX^®?

• 

  High silencing efficiency

We obtain >80% KD (Tebubio quality controls are performed by qPCR) leading to very low remaining gene expression in SilenciX®, compared to endogenous expression.

• KD stability - more than 500 days in culture

- Stable silencing over time (months) reinforced with hygromycine selection

• Syngenic Cell Lines

- No integration into the genome - vector remains anchored to the chromosomes giving access to
comparable genetic background between cell line control and knock-down.

• in vitro liposomal transfection

Catalogue or fully customized - there's a SilenciX^® suited to your needs...

The SilenciX technology is available as catalogue products (HeLa cells stably silenced for a gene of interest).
Or, depending on your needs, our laboratories can design and develop custom SilenciX cell lines from your cell lines.

What is in my ready-to-use SilenciX^® HeLa cell line kit?

• 1 vial of target specific SilenciX® cells
• 1 vial of control SilenciX^® cells (transfected with a non-relevant shRNA sequence)
• Specification sheets with quantatitive PCR quality controls
• Complete User Manual

Any questions? Get in touch