tebu-bio is a European company specialised in providing innovative reagents and laboratory services in Life Sciences

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Gene Editing - CRISPR-Cas9 genome editing reagents

All-in-one expressing vector

One vector expressing the CAS9 and the specific sgRNA with mCherry reporter to monitor the transfection efficiency.

The all-in-one vector can be delivered efficiently into cells with the help of our transfection reagent, CRISPR DNA-in.

The generated insertions and deletions can be revealed with a simple InDelCheck detection system based on T7-Endonuclease I assay. We can even design and provide the specific primer pairs.

CRISPR Donor for knock-in and knock-out selection

A Donor is required for knock-in and recommended for knock-out. We offer ready-to-use Donor vectors for each specific project. The vectors are also available in a cloning version.

Knock-in & knock-out for hard-to-transfect cells

Adenovirus is a means to efficiently deliver the CRISPR-CAS9 system into cells, even for hard-to-transfect cells. Furthermore, adenovirus provide transient expression and no integration in the genome. It's very well adapted to the CRISPR-CAS8 based genome editing.

We offer control adenovirus to monitor the efficiency of the delivery. Also, tailored adenovirus productions including the all-in-one and the donor vector.

Modified CAS9 mRNA and synthetic sgRNA

An approach without vectors can be also interesting. It was shown that 5MeC and PseudoU modifications on mRNA improve the translation and reduce the immune response. We provide ready-to-use CAS9 mRNA in 1mg/ml, as affordable catalogue products throughout Europe.

The gene-editing ribonuclear protein (RNP) complex is formed in the cells thanks to optimized translation. It was shown (Hendel et al 2015, Osborn et al 2016) that synthetic and modified sgRNA is highly preferable. The highlighted modification is 2'-O-methyl 3' phosphorothioate (MS) at both extremities of the sgRNA (red star).

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The transfection can be ensured by a dedicated reagent, CRISPR mRNA-in. And for those who would like to monitor transfection efficiency, there is also an eGFP mRNA similarly modified.

In Vitro Transcription sgRNA

Despite IVT sgRNA not being the most efficient ones, there are numerous articles using them. To help you with this, we provide plasmid expressing sgRNA under T7 promotor (see left) and a fast and robust in vitro transcription kit called T7-FlashScribe.

CAS9-expressing cell line and sgRNA libraries

When your work requires several, or recurrent, or even multiplex gene knock-out, a cell line model expressing CAS9 can be helpful. Notably, it is a smart way to explore the effects of loss of function in a pathway and why not make an epistatic study. Such screening is based on the delivery of a dedicated sgRNA libraries into the CAS9-expressing cell line.

Chromosome enumeration probes

Thanks to our VividFISH Chromosome FISH Probes, control the number of chromosomes and so alleles for specific site.