When doing WBs, IFs or IHCs, we normally pay a lot of attention to the primary antibody that should be chosen. We look at the datasheet, we check that the primary antibody has been validated for the species and application of our interest, we check if it has been used in publications, we look at the pictures in the datasheet… but sometimes we forget about the secondary.
Here are some tips to help you choose the best secondary. Because a good choice of a secondary antibody can be as important as choosing the right primary! Thanks to Camilo Moncada, Ph.D., from Rockland Inc, for his support!