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Research areas
    ADME-Tox
    Biomarkers
    Cell Biology and Signalling
    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
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Meet the authors
Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

PROTACs: A promising strategy in Drug Development

04/07/2019 by Frédéric Samazan No Comments
Proteasome degrading a protein (red) tagged with polyubiquitin

Thanks to its capability to recruit the natural protein degradation system of a cell, PROTACs (PROteolysis TArgeting Chimera) appears as a very promising technology in cancer therapy and disease treatment. As a distributor of innovative reagents for life science researchers, tebu-bio provides you with all the necessary tools to develop or test your own PROTACs system.

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Cell Biology and Signalling

Cell signalling: How to easily detect mono- and poly-Ub target proteins

26/02/2018 by Philippe Fixe, PhD No Comments
Recently, a new series of ready-to-use reagents for studying post-translation modifications (PTMs) of your proteins of interest have been released by Cytoskeleton, Inc. To illustrate the advantages of these research tools, this post is focuses on ubiquitination detection, which is known to be an important event in cell signalling.
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Cell Biology and Signalling

Check whether your protein is ubiquitylated!

11/10/2016 by Ali El Baya, PhD No Comments

Detecting ubiquitylation

Ubitest Flow Chart - LifeSensors at tebu-bio.com

Ubitest Flow chart – LifeSensors at tebu-bio.com

Determining the linkage of polyubiquitin on target proteins is challenging. The traditional methods are either through Mass Spectrometry or immunoblot with linkage specific antibodies, which are cumbersome. LifeSensors has developed the UbiTest assay,  a more definitive method for demonstrating the ubiquitylation linkage of a protein, which is to couple immunoprecipitation of polyubiquitylated protein with digestion by a linkage specific deubiquitylase prior to immunoblot analysis. An increased signal for the unmodified substrate or a decreased signal of polyubiquitylated substrate at high molecular weight after K48/K63 specific DUB treatment is a clear indication that the protein was K48/K63 ubiquitylated.

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Drug Discovery

Easily customizable E3-ubiquitin ligase assay kits

08/02/2016 by Ali El Baya, PhD No Comments

A ready-to-use E3 ligase activity enzymatic assay, the E3LITE kit, has been recently designed enabling researchers to screen for E3 enzyme inhibitors and potentially new therapeutical drugs. What’s it for and why is it of interest?

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