To date, detecting changes in the ubiquitylation of specific substrate proteins in response to external stimuli, e.g. stress, cytokine exposure, drug candidate treatment, etc., has been a long, labor-intensive process involving immunoprecipitation followed by gel electrophoresis and Western blot analysis. This method is low through-put, resource intensive, and only semi-quantitative at best.
ELISA like method to measure all ubiquitinated proteins in cell lysates
To enable researchers to avoid the above mentioned limitations, LifeSensors has developed the UbiQuantTM S kit as a facile, robust, and quantitative alternative to IP/WB analysis.