TFACT™ DNA-BINDING ELISA KITS » OCT2 TFACT™ DNA-BINDING ELISA by Assay Biotechnology. Source: tebu-bio.

Gene expression is regulated by different mechanisms. One of them is the binding of Transcription Factors (TF) to DNA sequences.

Traditionally, the study of TF-DNA interactions is made by several time-consuming and cumbersome: Electrophoretic Mobility Shift Assays (EMSA), Chromatin Immunoprecipitation, Western blotting, and expression of fused target and reporter genes.

ELISA-based formats now allow to have a more precise TF-DNA interaction study in addition to an ease of use.

FOX transcription factor family members regulate the expression of genes known to play a critical role in embryonic development and organ morphogenesis. These “helix-turn-helix” class of proteins display a common “forkhead” motif and are encoded by Forkhead genes. Recent lines of evidences suggest that FOX proteins are promising therapeutical targets and biomarkers in various areas. A good opportunity to review of the most reliable FOX primary antibodies for research applications!