When producing recombinant proteins, one can follow the purification process either by antibodies detecting the target protein specifically, or by adding a tag to it, thus using antibodies detecting not the protein itself, but the tag. This allows to have a battery of anti-tag antibodies that can be used with any protein, rather than having dozens of protein-specific antibodies. Our experts in recombinant protein production (either E. coli or HEK293) at our laboratories in France, are well aware of the key points in any of these processes.

It is not so straightforward to design an anti-tag antibody. One must be sure that it is able to recognise the tag, no matter what protein it is attached to. Here, peptide design and validation with multiple recombinant proteins having the same tag (either in the N-t or the C-t) is crucial. The reason why the anti-tag antibodies we have chosen perform so well in the hands of hundreds of researchers across Europe, is because we select them from sources that really follow this rule when producing their anti-tag antibodies. [Read more…]