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Research areas
    ADME-Tox
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    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Biomarkers

Where to find a sensitive Hyaluronic Acid (HA) quantitative assay

27/09/2020 by Agnès Audrain No Comments

Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid…) to resist to physical and mechanical constraints (torsion, flexion…). Over time, when HA production declines, tissues progressively lose these tensile properties, leading to wrinkles and fold, weak re-epithelisation and age-related troubles. But HA is also involved in many other chronic and cancer-related diseases. In this post, we’ll review one of the most popular HA quantification assays (ELISA test), known to be highly sensitive and robust, appreciated by researchers involved in cosmetology and drug discovery.

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Gene Expression - Molecular Biology, Headlines

Gel Filtration – Which column to choose for your size exclusion purification?

09/09/2016 by Isabelle Topin, PhD No Comments

Hand with Antibody V2-01Gel filtration (GF), also referred as Size Exclusion Chromatography (SEC), plays a key role in the high quality purification of enzymes, polysaccharides, nucleic acids, proteins and other biological macromolecules. Gel filtration is the simplest and mildest of all chromatography techniques to separate biomolecules on the basis of difference in size.  Nevertheless, the list of available columns is quite awesome and the characteristics of each of them are very different. Also, each characteristic highly influences the quality of the final purified product with important consequences in downstream applications.

I’ve put together a simple guide to help you find your GF system, together with some tips and tricks based on my experience when producing recombinant proteins and antibodies for our clients involved in the early R&D stages through to the latest phases of the bio-production flow.

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Download your copy of the guide “Gel Filtration – which column should you choose for your size exclusion purification?“

If you have any questions or if you need some help, leave a message below to get in touch with our lab experts!

Supplying Discovery Tools

3 tips for IHC troubleshooting

09/03/2016 by Ana Arraztio 1 Comment
Tips from being bio-reactive

The sensitivity and specificity of the primary and secondary antibodies used together with the IHC procedure used, are critical to avoid biased results. Several factors can cause false-positive or false-negative data, so they should all be verified as much as possible for each experimental set-up.

3 tips for IHC Troubleshooting - tebu-bio - being bio-reactive3 main experimental stages particularly need to be under control:

  • Detection of the antigen of interest by the primary antibody
  • Detection of the primary antibody by secondary antibodies
  • Tissue preparation

Here, let’s look at 3 tips that will be of help to improve your IHC data.

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Supplying Discovery Tools

Antibody purification troubleshooting tips

11/02/2016 by Ana Arraztio No Comments
Tips from being bio-reactive

Antibody-based techniques are widely used in Life Science laboratories. Antibody purification is often required to raise purity yield of antibody production batches or to reach publication-grade data. In this post, take advantage of some technical tips and troubleshooting points to improve your antibody purification.

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