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tebu-bio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
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    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
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Cell Biology and Signalling, Cell Sourcing - Cell Culture Technologies, Drug Discovery, Headlines, Supplying Discovery Tools

Get 1.8 fold more cardiomyocytes with MesoFate medium

30/09/2014 by Jean-François Têtu, PhD No Comments

The analysis of mouse and human embryonic stem cell differentiation cultures has indicated the existence of a cardiovascular progenitor, one of the earliest stages of mesoderm specification to the cardiovascular lineage. In this post, we’ll look, step-by-step, at a new cardiovascular differentiation experimental procedure starting from human pluripotent Stem Cells.

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Cell Sourcing - Cell Culture Technologies

FAQs on feeder cells for optimal human iPSC culture

05/09/2014 by Philippe Fixe, PhD No Comments
Tips from being bio-reactive

Human Newborn Foreskin Fibroblasts (NuFF cells) are genuine feeder cells for Stem cell research. Mitotically inactive NuFF cells (by irradiation or Mitomycin C chemical treatment) can efficiently maintain healthy undifferentiated human pluripotent cell status and support cell reprogramming. In this post, you’ll find the answers to Frequently Asked Questions collected by tebu-bio’s cell biology experts regarding these feeder layers.

I hope these FAQs will help you in your Stem cell research! Have a good read.

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News

RhoA/ROCK and F-actin modeling involved in Insulin secretion

21/08/2014 by Philippe Fixe, PhD No Comments
Research and Development, research applications

This is the conclusion of Liu X. et al. who demonstrated, in a mouse model, that RhoA/ROCK signaling pathway modulates insulin secretion of 3D cultured islet pancreatic ß-cells. This modulation is made through the regulation of Connexin 36.

Cell-permeable C3 transferase (C3T; Cytoskeleton Inc.) and the small molecule Y-27632 ROCK inhibitor (Stemgent-Asterand) were used in these studies.

External links regarding RhoA/ROCK in insulin secretion of pancreatic ß-cells

  • Liu X. et al. “Involvement of RhoA/ROCK in insulin secretion of pancreatic ß-cells in 3D
    Anti RhoA Human in WB analysis Cytoskeleton tebu-bio ARH03

    Western blot detection of RhoA (cat. nr ARH03) in cell extracts (50 µg each) of rat NRK cells (lane 1), human HeLa cells (lane 2), bovine brain extract (lane 3), and human platelet cell extract (lane 4).

    culture” (2014) Cell Tissue Res. DOI: 10.1007/s00441-014-1961-2

  • Cell permeable C3 transferase (cat. nr CT04), inhibits cellular Rho within 2-4h
  • Selective Rho Kinase (ROCK) inhibitors and anti Human RhoA antibody (a Mouse monoclonal that only recognizes RhoA, not RhoB, RhoC, Rac1, Rac2, Rac3, Cdc42 or H-Ras).

I hope you will enjoy this new publication. Don’t hesitate to leave your comments or remarks regarding the use of bioactive small molecules in signal transduction studies.

Cell Sourcing - Cell Culture Technologies, Stem Cells

Cell reprogramming strategies for Stem cell research & cell sourcing

05/08/2014 by Philippe Fixe, PhD No Comments

Cellular reprogramming is a process by which a fully differentiated cell type with specialized functions, is brought to be transformed into another cell type. This reprogrammed cell type exhibits different characteristics and functions it would not display initially under normal physiological conditions.

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