Since the discovery of reprogramming factors in 2006 and the boom of CRISPR gene editing strategies, induced pluripotent stem cells (iPSC) have emerged as new cellular models. The development of 3D cell culture technologies has also contributed to the generation of induced Pluripotent Stem Cell (iPSC) derived cells, with unique applications from patient-specific drug responses testing, to regenerative medicine. I would like to introduce in this post a selection of reagents in this domain, a combination of both routine and innovative quality reagents, that I consider as bringing something extra to your stem cell research projects.
Stauprimide is known to prime Embryonic Stem Cells (ESC) by targeting the c-Myc-activating transcription factor NME2. Its mechanism of action is linked to the inhibition of the nuclear localization of NME2 leading to the downregulation of the transcription of the c-myc oncogene.
In a recent study, Bouvard C. et al. evidenced that Stauprimide’s mechanism of action could also be used to pharmacologically targetc-myc transcription in cancers.Â [Read more…]
StemBeadsÂ® FGF2 is a revolutionary growth factor supplement that offers a more efficient way to grow FGF2 dependent stem cell cultures.
This supplement delivers a steady release of growth factor into your media of choice creating a more stable environment allowing for:
- Reduction of media changes by 67%
- Better culture quality through reduction of spontaneous differentiation
- No change of culture conditions – use your favourite media
I hear you thinking “Well, that’s just more marketing promises… “. Well, it isn’t, it’s real life!
Just read on and see some of the feedback from researchers… [Read more…]
New techniques such as cDNA microarrays have enabled us to analyse global gene expression. However, almost all cell functions are executed by proteins, which cannot be studied simply through DNA and RNA techniques.Â In fact, experimental analysis clearly shows disparity can exist between the relative expression levels of mRNA and their corresponding proteins (1).
Therefore, analysis of the proteomic profile is critical, especially in processes that rely on secreted proteins (e.g. inflammation).Â The conventional approach to analysing multiple protein expression levels has been to use 2-D SDS-PAGE coupled with mass spectrometry. However, these methods are slow, expensive, labor-intensive and require specialised equipment.Â Moreover, these traditional methods of proteomics are not sensitive enough to detect most secreted biomarkersÂ (typically at pg/ml concentrations).
For some years now, antibody arrays have been available to study markers and publish their discoveriesÂ in various areas likeÂ Immunology, Atherosclerosis, Inflammation, Angiogenesis, Immunoediting and even signaling pathways (ex. phosphorylation, Receptor Tyrosine Kinases…). So far, however, and in spite of the growing demand by researchers working on stem cells, there were no antibody arrays for this area of research, meaning that individual Western Blots had to be performed. But not any more! [Read more…]
Peroxisome proliferator-activated receptor alpha (PPAR-alpha also known as NR1C1) Â regulates a myriad of biological processes. It is a key modulator of lipid metabolism.
Vergori, L. et al.Â have shown in murine modelsÂ how PPAR-alpha regulates endothelial progenitor cell maturation and myeloid lineage differentiation via a NADPH oxidase-dependent mechanism. (1) All the data described in this publication suggest that PPAR-alpha, in murine models, is a critical regulator of recruitment, homing and maturation of Bone Marrow-derived progenitor cells. [Read more…]
Cord blood might be a much-needed solution.Â
Few diseases stump researchers as much as Alzheimerâ€™s disease. Quite simply, nothing has worked beyond some symptomatic relief. On top of that, scientists are still uncertain about what exactly causes the disease. For example, are the characteristic beta-amyloid plaques in the brain a cause of Alzheimerâ€™s disease â€” or a consequence? Any promising results are welcome to a community used to setbacks. [Read more…]
The behavior of adult mammary stem cells (MaSCs) is precisely controlled by the activities of hormones and local factors, though the underlying mechanisms remain obscure. A recent report by Cai et al in Genes & Development illustrates the dynamic interaction between systemic ovarian hormones, Wnt signaling, and the Wnt agonist R-spondin1 (Rspo1) to promote MaSC self-renewal.
In response to estradiol and progesterone, R-spondin1 and Wnt4, but not Wnt7B, act as niche factors to drive MaSC regeneration, with Wnt4 acting through the canonical Wnt/Î˛-catenin signaling pathway. This work establishes a clear mechanistic link between locally acting Wnt signals and the systemic hormone growth response of MaSCs, unveiling the intriguing concept that hormones induce a collaborative local niche environment for stem cells.
Working on unveiling the mechanisms of stem cell fate and differentiation? We’d like to hear from you!
Reprogramming-qualified B18R Recombinant Protein, Carrier-free is of interestÂ for cellular reprogramming and other applications requiring RNA-mediated gene delivery.Â B18R protein is a Vaccinia virus-encoded receptor with specificity for mouse, human, rabbit, pig, rat and cow Type 1 interferons and has potent neutralizing capability acting asÂ a decoy receptor for Type 1 interferons.
- Stemfactor B18R Recombinant Protein, Carrier-free bioactivity is demonstrated by the neutralization of IFN-alpha or IFN-beta effects in an assay of IL-12 production from CD40L-activated monocytes.
- The biological function is validated by down-regulation of gene expression by qRT-PCR of TLR3, IFIT1, and OASI in response to B18R treatment on human fibroblasts to levels equivalent to non-transfected cells.
- The efficiency of mRNA reprogramming is tested by performing mRNA reprogramming with OKSML on neonatal foreskin fibroblasts and demonstrating greater than 0.5% iPSC colony formation.
Previously only available as part of the mRNA Reprogramming Kit or microRNA Booster Kit,Â Reprogramming-qualified B18R Recombinant Protein, Carrier-free is now available individually.
Traditionally, Fetal Bovine Serum (FBS) has been used as an additive to cell culture media for the in vitro growth of pluripotent stem cells. FBS is an inherently variable component of the cell culture system, so consistent performance demands extensive screening of manufactured lots. To circumvent this problem, companies began offering â€śstem cell qualifiedâ€ť serum. One issue with this solution is that the qualification is not always performed on a stem cell line relevant to the researcher’s own cell lines. Years later, researchers began using Life Technologies KnockoutÂ® Serum Replacement (KOSR) to grow and maintain undifferentiated human embryonic stem (ES) cells in culture. While KOSR has shown to be more stable than FBS and performs better in maintaining undifferentiated ES and induced pluripotent stem (iPS) cells, it is expensive and many researchers are today looking for a more cost effective media solution for their pluripotent stem cell culture. To address this need in the scientific community, GlobalStem now offers a more economical solution with theirÂ new PluriQâ„˘ Serum Replacement. [Read more…]
Self-renewal and differentiationÂ capacitiesÂ into multiple cell-types are 2 main characteristics of Stem Cells. ContrarilyÂ to Embryonic Stem Cells, Adult Stem Cells (ASC) are already committed to a differentiated lineage. Nevertheless, ASC still retain capacities of generating various cell types within their restricted lineage. It’s thusÂ crucial to be able to access high quality and reliable research tools when tracking cell differentiation. Here, we’ll review the most popular reagents used to characterize the ectodermal lineage.