For about 5 years now, there has been a renewed interest for mRNA molecules and their numerous applications. Mature mRNAs can be transfected into cells (ex. in mammalian cells) for various purposes (ex. cell reprogramming and iPSCs production, genome editing (such as CRISPR-CAS9), cell line engineering (eg. protein production)) without the need for cloning strategies in expression vectors. In addition, mRNA molecules are now becoming a promising elements in Drug Discovery to deliver genetic information and mRNA-based therapeutics in cells.
Whatever the application used, mRNA production by in vitro transcription (IVT) is tricky and deserves high quality enzymes. In this post, let’s take a closer look at the mScript technology for optimized mRNA productions.