Mass spectrometry is a technology which is widely used in most scientific disciplines that require accurate and precise measurement of elemental and molecular components. Its use in the pharmaceutical sector is often associated with the Drug Discovery and Development process. The primordial step to analyze a sample is to perform a sample treatment to enable its study. It really requires know-how to obtain good quality results. The following tips and tricks can help you to rapidly select a protein purification strategy and to anticipate some possible problems.
Decidedly, producing and purifying a protein is a form of art.
Indeed, each recombinant protein (rec. protein) to be produced in vitro has specific characteristics and singularities that make its production and purification most definitely challenging. In this post, I’d like to share and review a few tips and basics for optimal design of experiment when producing functional recombinant proteins and working on protein purification.
Monoclonal antibody (mAb) engineering and their development as therapeutic or diagnostic tools have become the must in pharmaceutical industry, in medical biotech and in the research world. Purification of this type of biosimilar requires a particular know-how.
Because of their rapid growth and their affordability, bacterial expression systems are very convenient when producing recombinant proteins.
Nevertheless, one of the key elements in the design of the experimental procedures for producing a recombinant protein in¬ E. Coli¬†is the selection of the right bacterial strain for the right protein. This is not an easy step but remains of utmost importance.
Based on our¬†in-house¬†experience, we’ve compiled a guide¬†aimed at helping you in your choice of¬†the ‚Äúideal‚ÄĚ engineered bacterial strain for efficient recombinant proteins expression.
You’ll find useful¬†information¬†for selecting the reliable strains suited to your downstream applications, with characteristics (quality, biological activity, folding‚Ä¶).
Interested?¬†Download your copy¬†here:
Guide to choosing the best bacterial strains for recombinant protein expression
Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so¬†there shouldn’t be any¬†need to look for¬†alternatives such as¬†custom productions.
So the question is, why should you prefer a special production?
Well, there are several reasons (and they’re good ones too!). [Read more…]