Mass spectrometry is a technology which is widely used in most scientific disciplines that require accurate and precise measurement of elemental and molecular components. Its use in the pharmaceutical sector is often associated with the Drug Discovery and Development process. The primordial step to analyze a sample is to perform a sample treatment to enable its study. It really requires know-how to obtain good quality results. The following tips and tricks can help you to rapidly select a protein purification strategy and to anticipate some possible problems.
Decidedly, producing and purifying a protein is a form of art.
Indeed, each recombinant protein (rec. protein) to be produced in vitro has specific characteristics and singularities that make its production and purification most definitely challenging. In this post, I’d like to share and review a few tips and basics for optimal design of experiment when producing functional recombinant proteins and working on protein purification.
Monoclonal antibody (mAb) engineering and their development as therapeutic or diagnostic tools have become the must in pharmaceutical industry, in medical biotech and in the research world. Purification of this type of biosimilar requires a particular know-how.
Because of their rapid growth and their affordability, bacterial expression systems are very convenient when producing recombinant proteins.
Nevertheless, one of the key elements in the design of the experimental procedures for producing a recombinant protein inÂ E. ColiÂ is the selection of the right bacterial strain for the right protein. This is not an easy step but remains of utmost importance.
Based on ourÂ in-houseÂ experience, we’ve compiled a guideÂ aimed at helping you in your choice ofÂ the â€śidealâ€ť engineered bacterial strain for efficient recombinant proteins expression.
You’ll find usefulÂ informationÂ for selecting the reliable strains suited to your downstream applications, with characteristics (quality, biological activity, foldingâ€¦).
Interested?Â Download your copyÂ here:
Guide to choosing the best bacterial strains for recombinant protein expression
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