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Acting and reacting in life science biotechnologies

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2 new validated anti-PGP 9.5 antibodies for neuroscience research

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Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase L1 (UCH-L1)) has become an accepted biomarker for neurons and cells of the diffuse neuroendocrine system (DNES) but also when studying neurodegenerative disorders (e.g. Alzheimer’s and Parkinson’s diseases) and cancers.

Research antibodies specific for PGP9.5 / UCH-L1 stain neuronal cell bodies and axons in the central and peripheral nervous systems, small nerve fibres in nerve tissue, neuroendocrine cells in the pituitary, thyroid pancreas, and also tumours of the DNES.

In this context, Cedarlane just release 2 new anti-PGP9.5 / UCHL1 rabbit polyclonal antibodies fully validated & guaranteed for IHC analysis of Paraffin Embedded Sections, IF and WB applications on Human, Rat and Mouse tissues. [Read more…]

10 Mitochondrial marker antibodies

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There is a high demand for selective fluorescent probes for the imaging of particular organelles and biomarkers in living cells. This is particularly the case for mitochondrial research. In this post, we will focus on another type of powerful research tools (ie. primary antibodies) that have been proven to be very useful for studying mitochondria. The 10 antibodies highlighted here have been successfully used by our clients for immunoblotting of mitochondrial proteins and are all covered by tebu-bio’s Satisfaction Guaranteed.

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Flow cytometry intracellular staining

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Intracellular Flow Cytometry enables the identification and analysis of signaling and functional markers within cells from samples containing heterogeneous cell populations. Many cell types can be thus identified through their intracellular Flow Cytometry patterns and key intracellular signaling pathways and their respective post-translational modifications can be conveniently analysed. Behind these unique performances, Intracellular Flow Cytometry require anyhow optimized immunoreagents for cell permeabilization, intracellular staining and fluorescent detection. [Read more…]

7 popular phospho-specific antibodies

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Whatever the research domain (oncology, cardiovascular diseases, Diabetis/obesity, inflammation, autoimmune disorders…), phosphorylation study is essential when looking for activation of cell signaling pathways. In 2014, we strived to bring researchers practical technical tips to obtain reliable results when analysing phospho-proteins. In this post, we’ll be taking a look at 7 popular and robust phospho-specific antibodies.

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Zebrafish – an ideal model for morphogenesis analysis

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Cdh1 antibody - Rabbit Polyclonal antibody to E-cadherin (Gentex | tebu-bio)

E-Cadherin (Cdh1) expression in Zebrafish with Genetex antibody (GTX125890 | tebu-bio)

In a recent study, Xiong F. et al. have developed a robust Zebrafish-based model allowing the analysis of the impact of cell shape and division orientation on epithelial development. This model explores possible links between cell shape and division orientation in epithelial diversity.

Among all the research tools used, the team of scientists investigated Cdh1 expression by IHC-Wm with a Genetex antibody, dedicated to Zebrafish studies (cat. nr GTX125890). This unique antibody is available in Europe thorugh tebu-bio for IHC applications (recommended dilutions 1:100-1:1000) including IHC Whole mount (IHC-Wm).

This emphasizes the need for researchers to have access to high quality and validated antibodies focused on Zebrafish model, as described in a previous post, showing the benefits of using such primary antibodies in Zebrafish whole mount IHC staining.
Source:
Xiong F. et al. “Interplay of Cell Shape and Division Orientation Promotes Robust Morphogenesis of Developing Epithelia” Cell Volume 159, Issue 2, p415–427. DOI: http://dx.doi.org/10.1016/j.cell.2014.09.007

3 monoclonals to discriminate free Ubiquitin from linear and polyubiquitin chains

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Ubiquitin (Ub) is an 8 kDa  highly conserved polypeptide, commonly expressed in eukaryotic cells. Ub is added to lysine residues of the target proteins. This post-translational modification (known as ubiquitination) is made through the sequential action of 3 enzymes (E1 Ub activating enzyme, E2 conjugating enzyme and E3 ligase).

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PARP and Top1 inhibitors most beneficial in endonuclease-deficient cancer cells

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The synergistic combination of Poly(ADP-ribose) polymerases (PARP) and Topoisomerase I (Top1) inhibitors most beneficial on endonuclease-deficient cancer cells in cancer therapies ?

This is one of the elements discussed in the recent publication by Dr Benu Brata (National Institutes of Health (USA) and Indian Association for the Cultivation of Science (India) in Nucleic Acids Research. (1)

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5 reliable research tools to study ERG, USP9X and DUB inhibitor in prostate cancer

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In a recent paper published in PNAS, Wang et al. show that the inhibition of the deubiquitinase USP9x by the small molecule WP-1130 is able to induce degradation of the transcription factor E-twenty-six related gene (ERG).

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2 cost-effective antibody options for Hypoxia pathway analysis

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Low oxygen tension, or Hypoxia, regulates numerous cellular and tissular functions. In cancer research, hypoxia is a key regulator of tumor development, aggressiveness and therapy resistance by acting on malignant cells and their microenvironment. Hypoxia is also involved in age-related diseases and acts through intracellular and intercellular cascade of events (exosomes, paracrine loops, angiogenesis…) (1-2). [Read more…]