Signal-Seeker assays (Cytoskeleton Inc.) provide researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs) of your Protein of Interest.
In this post, I invite you to look at experimental results obtained with these unique assays for the characterization of Tyrosine Phosphorylation, Ubiquitination, SUMOylation and Acetylation of endogenous proteins.
In a recent paper, Horita H. et al. identified a set of novel Post-Translational Modifications (PTMs) of the protein Programmed Cell Death Ligand (PD-L1).
Profile of PD-L1 post-translational modifications and their roles in regulating PD-L1 protein levels. Source: Horita H. et al. (2017) – http://dx.doi.org/10.1016/j.neo.2017.02.006 (Cytoskleton Inc).
For this, a novel series of PTM enrichment assays enabling the highly specific profiling of 4 key PTM profiles (Tyrosine phosphorylation (pY), Acetylation (Ac), Unbiquitination (Ub) and SUMOylation 2/3), have been used on EGF-stimulated A431 cell line. They revealed that EGF induced pY, Ac, mono- and poly-Ub Of PD-L1 in these EGF treated epidermoid carcinoma cells. The authors also suggested that the balance between mono- / poly-Ub of PD-L1 might regulate PD-L1 stability, as already described with LDB-1 protein.
These novel PD-L1 PTMs and their related regulatory actions might be valuable information in the future for the design of drug strategies targeting PD-L1/PD-1 immune checkpoint inhibition.
