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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
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  • Meet the authors
Cell Biology and Signalling

How to make Post Translational Modification characterization easier

10/04/2018 by Frédéric Samazan No Comments

Signal-Seeker assays (Cytoskeleton Inc.) provide researchers with the most comprehensive reagents enabling highly sensitive and specific detection of the key Post Translational Modifications (PTMs) of your Protein of Interest.

In this post, I invite you to look at experimental results obtained with these unique assays for the characterization of Tyrosine Phosphorylation, Ubiquitination, SUMOylation and Acetylation of endogenous proteins.

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Supplying Discovery Tools

3 novel PD-L1 post-translational modifications identified

18/04/2017 by Philippe Fixe, PhD No Comments

In a recent paper, Horita H. et al. identified a set of novel Post-Translational Modifications (PTMs) of the protein Programmed Cell Death Ligand (PD-L1).

Model: Profile of PD-L1 post-translational modifications and their roles in regulating PD-L1 protein levels.

Profile of PD-L1 post-translational modifications and their roles in regulating PD-L1 protein levels. Source: Horita H. et al. (2017) – http://dx.doi.org/10.1016/j.neo.2017.02.006 (Cytoskleton Inc).

For this, a novel series of PTM enrichment assays  enabling the highly specific profiling of 4 key PTM profiles (Tyrosine phosphorylation (pY), Acetylation (Ac), Unbiquitination (Ub) and SUMOylation 2/3), have been used on EGF-stimulated A431 cell line. They revealed that EGF induced pY, Ac, mono- and poly-Ub Of PD-L1 in these EGF treated epidermoid carcinoma cells. The authors also suggested that the balance between mono- / poly-Ub of PD-L1 might regulate PD-L1 stability, as already described with LDB-1 protein.

These novel PD-L1 PTMs and their related regulatory actions might be valuable information in the future for the design of drug strategies targeting PD-L1/PD-1 immune checkpoint inhibition.

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Cell Biology and Signalling

Protein post-translational modification analysis

28/04/2016 by Ali El Baya, PhD No Comments
Protein and post-translational modifications

Protein post-translational modifications (PTMs) are part of a complex regulatory network that controls physiological and pathological cellular processes. In this post, new user-friendly assays are introduced to help Life Scientists gain a deeper understanding of these mechanisms involved in protein biology.

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