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Research areas
    ADME-Tox
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    Cell Sourcing - Cell Culture Technologies
    Drug Discovery
    Gene Expression - Molecular Biology
    Stem Cells
    Supplying Discovery Tools
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Tebubio's blog - Acting and reacting in life sciences and biotechnologies
  • Home
  • Research areas
    • ADME-Tox
    • Biomarkers
    • Cell Biology and Signalling
    • Cell Sourcing – Cell Culture Technologies
    • Drug Discovery
    • Gene Expression – Molecular Biology
    • Stem Cells
    • Supplying Discovery Tools
  • Contact us
  • Meet the authors
Supplying Discovery Tools

How can TR-FRET facilitate your cellular studies?

06/01/2020 by Frédéric Samazan No Comments
Inflammatory Cytokine Array and ELISA Services tebu-bio

Time-Resolved Förster Resonance Energy Transfer (TR-FRET) is a robust and homogeneous fluorescence detection technology and a real alternative to classical ELISA and Western Blot to perform Cell Signalling studies. Based on this improved TR-FRET technology, tebu-bio is pleased to introduce a new product line of high-quality, fully-validated and affordable kits. These ready-to-use Thunder™ kits enable sensitive, simple and rapid measurement of low amounts of specific intracellular phosphorylated and total proteins in cell lysates from adherent or suspension cells.

The main features of these new Thunder™ TR-FRET kits are presented in this post.

How does it work?

Even though based on the traditional immuno-assay sandwich principle, the kits use a streamlined protocol wherein the antibody-target sandwich complex is formed in solution in a single addition and incubation step, without any wash steps. This protocol dramatically decreases hands-on time (5 minutes) and enables faster time to results (1-4 hours).

Figure 1:TR-FRET principle

After lysis steps, a pair of antibodies specific for the target protein is added to the lysate sample (Fig.1). One antibody is labeled with a donor fluorophore (a Europium chelate), while the second antibody is labeled with an acceptor fluorophore (a far-red dye). Upon excitation of the Europium chelate at 320 or 340 nm, energy is transferred from the donor to the acceptor fluorophore if they are sufficiently close (within 10 nm) for FRET. This results in the emission by the acceptor of a TR-FRET signal at 665 nm.

Why is THUNDER TR-FRET a smart solution for cellular studies?

OPTIMAL ANTIBODY PAIR SELECTION

  • Validated antibodies were selected to guarantee specificity for the target protein.
  • Many donor/acceptor fluorophores were tested to select the optimal pair providing the best TR-FRET signal-to-background.
  • Extensive testing of labeled antibody pairs using optimized lysis buffers followed by assay optimization were conducted to ensure specificity, reproducibility and optimal TR-FRET assay performance.

RIGOROUS VALIDATION

All Thunder™ kits are subjected to a stringent validation process using lysates from cells that are treated with pathway-specific activators and inhibitors to further confirm target specificity.

CONFIRMED LOT-TO-LOT CONSISTENCY

All Thunder™ kits are developed, validated and manufactured to ensure lot-to-lot consistency using a functional quality control assay where each new lot is compared to the previous lot.

AFFORDABILITY

Thunder™ is an affordable cell-based assay platform that provides easy access to the TR-FRET technology for all researchers looking to quantify low amounts of endogenous proteins in cells.

Assay workflow

All it takes is 3 simple steps to complete the workflow of all the TR-FRET Cell Signaling Assays.

Assays can be run using a 2-plate (transfer) protocol:

Or a 1-plate (all-in-one-well) protocol:

Assays are optimized to be run in 96-well or 384-well plates using the same total volume (20µL).

Typical validation data

Figure 2:Modulation of Phospho-IRβ (Y1150/Y1151) in B lymphocytes

Most popular targets

  • AKT (pan)
  • EGFR : Epidermal Growth Factor
  • STAT6 : Signal Transducer and Activator of Transcription 6
  • IL-2 & IL-6: Human Interleukin 2 & 6
  • IFNγ : Human Interferon Gamma
  • cAMP : adenosine 3′,5′-cyclic monophosphate

Discover all THUNDER kits available

  • Cell Signalling Assay Kits : Simple and rapid detection of either a specific phosphorylated target protein (Phospho-protein kit) and/or total protein in cell lysates (Total protein kit)
  • Biomarker Assay Kits : Assay developed to easy and quickly quantitate pro-inflammatory human cytokines in cell supernatants
  • cAMP Assay Kits : Assay kit provide a simple, rapide, robust and affordable tool to measure intracellular cAMP changes following G-protein coupled receptor (GPCR) activation.

Try a real alternative to perform your cell signalling studies in a different way!

Contact us to discuss your project
Supplying Discovery Tools

Cell Signalling – how is the Phospho Explorer antibody array used?

14/11/2018 by Isabelle Nobiron, PhD No Comments
Phospho Arrays - Full Moon - tebu-bio

If your research interest is focused on the identification of cell signalling pathways and protein phosphorylation profiles, the comprehensive Phospho Explorer Array may be of great help to you, measuring changes in phosphorylation status in more than 1000 targets at once. I invite you to take a look at the technology and its benefits, after which I’ve included some publications in 2018 to give you an idea of how other researchers have used this array and how it helped them generate meaningful data in their phosphorylation studies.

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Biomarkers

Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection

17/04/2018 by Frédéric Samazan No Comments
CytoGlow Cell-Based (Phospho) ELISA kits Assay Biotech tebu-bio

Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.

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Supplying Discovery Tools

Cell Signalling: Ras-Raf-MEK-ERK pathway research tools

07/07/2017 by Isabelle Nobiron, PhD No Comments

The Ras-Raf-MEK-ERK Pathway is an important cell signalling route with many implications for cancer biology and therapeutic development. Dis-regulation of some of this pathway’s proteins expression and phosphorylation status are observed in about one-third of all human cancers. Access to specific tools to study this pathway is essential to a better understanding of its role in cancer for novel drug development. If you are working on this topic, you’ll be interested in taking a look at the range of reagents on offer for a wide variety of applications.

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