PD-L2

Screen for inhibitors of PD-1 signalling with a complete cellular assay system

28/10/2016 By Ali El Baya, PhD Leave a Comment

Immunotherapy represents a field in Drug Discovery which is quickly developing and leading to significant progress in treatments of a number of diseases, especially cancer. The approach is based on inducing, enhancing, or surpressing an immune response. Therapeutic manipulation of immunopathways has led to promising clinical results . The first therapeutic antibodies directed against the checkpoint receptor PD-1 have been already brought to the market (Nivolumab, Pembrolizumab) by Bristol Myers Squibb and Merck/MSD respectively, and approved for the treatment of diverse cancer types.

Today, I would like to review tools to build up a comprehensive assay set up for cell based inhibitor screening on PD-1 / PD-L1/PD-L2 binding. [Read more…]

Cancer Immunotherapy: Generate your PD-L1 target cell line

06/04/2016 By Ali El Baya, PhD Leave a Comment

Fig 1: Immuno checkpoint proteins on T cell interact with ligands on APCs or cancer cells

In a recent post, I invited you to discover a cell line provided by BPS Bioscience over-expressing the checkpoint receptor PD-1. They have now just released a new kit which allows to generate a PD-L1 and TCR activator over-expressing target cell line. [Read more…]

How to screen for inhibitors of PD-1 signalling in a cellular assay

09/02/2016 By Ali El Baya, PhD Leave a Comment

Fig 1: Immuno checkpoint proteins on T cell interact with ligands on APCs or cancer cells

The treatment of diseases by inducing, enhancing, or surpressing an immune response is referred to as Immunotherapy. Therapeutic manipulation of immunopathways has led to promising clinical results for the treatment of a number of diseases. The first therapeutic antibodies directed against the checkpoint receptor PD-1 have been already brought to the market (Nivolumab, Pembrolizumab) by Bristol Myers Squibb and Merck/MSD respectively, and approved for the treatment of diverse cancer types.

Today, I would like to review a versatile tool to conduct cell based inhibitor screenings on PD-1 / PD-L1/PD-L2 binding. [Read more…]

9 pathway-specific screening assays in Immunotherapy

02/07/2015 By Ali El Baya, PhD Leave a Comment

The immune system is a system of cells and organs whose function is to defend an organism from foreign pathogens. With the ability to mount a response against virtually any foreign material and return to a quiescent state following neutralization of the threat, this fascinating organ system displays remarkable specificity and plasticity. To achieve this, there is a multifaceted balancing act between the many activators and suppressors which maintains homeostasis of the body’s perhaps most complex organ system.

[Read more…]