Gastrointestinal (GI) primary cells represent a powerful approach for the in vitro study of the physiopathology of this unique tissue. Researchers have now identified intimate interactions between GI cells and their microbia with neurological disorders pointing out the need to access highly characterized sources of GI primary cells to design reliable and more physiologically relevant in vitro cellular models.

Continue reading

A ready-to-use E3 ligase activity enzymatic assay, the E3LITE kit, has been recently designed enabling researchers to screen for E3 enzyme inhibitors and potentially new therapeutical drugs. What’s it for and why is it of interest?

First at all, a quick reminder… Cytokines play important roles in inflammation, innate immunity, angiogenesis and cell growth. They are involved in a lot of disease processes like cancer, obesity and inflammatory and neurobiological diseases.

The RayBio® Membrane-Based Antibody Arrays (C-Series) are tools for screening and comparing expression levels of many cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample type.

After these brief definitions, today I would like to introduce you to a new tool allowing multiple cytokine detection. RayBio C-series Neurobiological Discovery Arrays will help you to measure several factors in inflammation, immunology. This method is very popular and can be used for two species: human and mouse.

Analysis of neuronal cell fates and biological activities sometimes relies on the effective staining of a protein of interest (a.k.a. marker) with a specific primary antibody validated for immuno-assays. These markers are used for labeling specific neuron lineages and understand their roles in physiological and various neurological disorders (e.g. Alzheimer’s disease, Parkinson’s disease and Amyotrophic lateral sclerosis). The choice of guaranteed antibodies is crucial. In this post, a selection of the most popular primary antibodies used in neuroscience research and used by researchers all over the world is made. This selection of high quality research antibodies is illustrated with experimental results obtained from skilled scientists.